Description

3'',5''-Bis-o-T-Butyldimethylsilyl N4,5-Dimethyldeoxycytidine is a sophisticated, high-purity nucleoside derivative specifically designed for advanced oligonucleotide synthesis. This compound is a critical protected intermediate, enabling the precise construction of modified DNA sequences for research and therapeutic applications. It is essential for pharmaceutical R&D, biotechnology, and academic laboratories focused on antisense oligonucleotides, siRNA, and other nucleic acid-based modalities.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): Serves as a key phosphoramidite building block for the automated synthesis of modified DNA strands.
• Antisense Oligonucleotide (ASO) Development: Used in the research and production of therapeutic ASOs targeting genetic diseases.
• siRNA and miRNA Synthesis: A crucial precursor for creating chemically stabilized small interfering RNA and microRNA constructs.
• Diagnostic Probe Manufacture: Enables the production of labeled and modified nucleic acid probes for molecular diagnostics and hybridization assays.
• Medicinal Chemistry & Drug Discovery: Facilitates the exploration of structure-activity relationships (SAR) in nucleic acid-based drug candidates.
• Academic & Institutional Research: Supports fundamental studies in biochemistry, molecular biology, and genetics requiring custom oligonucleotides.

Basic Information

Product Name	3'',5''-Bis-o-T-Butyldimethylsilyl N4,5-Dimethyldeoxycytidine
CAS No.	2062540-26-3
Molecular Formula	C23H45N3O4Si2
Molecular Weight	483.80 g/mol
Synonyms	5'-O,3'-O-Bis(tert-butyldimethylsilyl)-N4,5-dimethyl-2'-deoxycytidine; N4,5-Dimethyl-5'-O,3'-O-bis(tert-butyldimethylsilyl)-2'-deoxycytidine; 3',5'-Bis-O-(tert-butyldimethylsilyl)-N4,5-dimethyldeoxycytidine; TBDMS-protected N4,5-dimethyldeoxycytidine; N4,5-Dimethyl-2'-deoxycytidine 3',5'-bis-TBDMS ether; Protected N4,5-dimethyldeoxycytidine; dC(N4-Me,5-Me) 3',5'-bis-TBDMS
EINECS	Contact for details

Quality Control

Our production of 3'',5''-Bis-o-T-Butyldimethylsilyl N4,5-Dimethyldeoxycytidine adheres to stringent quality protocols suitable for pharmaceutical intermediate applications. Every batch is characterized and tested using advanced analytical techniques including HPLC, NMR, and mass spectrometry to ensure identity, purity, and consistency. Certificates of Analysis (COA) with detailed chromatographic data are provided to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label or COA. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions. For long-term storage, consider storing under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.