Description

n-(Azido-Peg3)-n-Bis-(Peg3-T-Butyl Ester) is a specialized heterobifunctional polyethylene glycol (PEG) derivative featuring an azide group and two carboxylic acid-protected termini. This compound matters as a critical building block in bioconjugation and click chemistry, enabling the precise, high-yield linkage of biomolecules, polymers, and surfaces under mild, aqueous conditions. It is essential for researchers and manufacturers in pharmaceutical development, particularly for creating antibody-drug conjugates (ADCs), peptide therapeutics, and advanced drug delivery systems, as well as in materials science for surface functionalization.

Application

• Bioconjugation Linker: Serves as a key reagent for coupling peptides, proteins, or antibodies to drugs, dyes, or other polymers via strain-promoted azide-alkyne cycloaddition (SPAAC) click chemistry.
• Antibody-Drug Conjugate (ADC) Synthesis: Used to construct the PEG-based linker-payload component, improving solubility and pharmacokinetics of the final therapeutic.
• Polymer and Dendrimer Functionalization: Enables the modular assembly and modification of polymeric structures for tailored material properties.
• Surface Modification and Immobilization: Facilitates the covalent attachment of bioactive molecules to nanoparticles, biosensors, and diagnostic assay platforms.
• Proteomics and Chemical Biology Tools: Acts as a probe or crosslinker for labeling and studying protein interactions and cellular pathways.
• Hydrogel Formation: Participates in the cross-linking of polymer networks to create biocompatible matrices for tissue engineering and 3D cell culture.

Basic Information

Product Name	n-(Azido-Peg3)-n-Bis-(Peg3-T-Butyl Ester)
CAS No.	2055042-56-1
Molecular Formula	C31H59N3O12
Molecular Weight	665.82 g/mol
Synonyms	1-Azido-3,6,9,12,15,18,21,24-octaoxahexacosan-26-oic acid, 26-[(1,1-dimethylethoxy)carbonyl]-27-[(1,1-dimethylethoxy)carbonyl]-; Azido-PEG3-acid, bis(t-butyl ester); N3-PEG3-T-Butyl Ester; 1-Azido-3,6,9,12,15,18,21,24-octaoxahexacosane-26,27-dioic acid bis(1,1-dimethylethyl) ester; Heterobifunctional PEG Azide t-Butyl Ester; Azido-PEG3-t-butyl ester crosslinker; 2055042-56-1
EINECS	Contact for details

Quality Control

Our n-(Azido-Peg3)-n-Bis-(Peg3-T-Butyl Ester) is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via NMR and Mass Spectrometry, to ensure it meets the high standards required for research and pharmaceutical applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Colorless to pale yellow viscous liquid or oil
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solvent Residue (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.