Description

Azido-PEG6-NHS ester is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring an amine-reactive N-hydroxysuccinimide (NHS) ester at one terminus and a click-chemistry compatible azide group at the other. This compound is critical for creating stable, site-specific conjugations between biomolecules, such as proteins or peptides, and other azide- or alkyne-functionalized entities. It is primarily utilized by researchers and manufacturers in the fields of bioconjugation, proteomics, and the development of antibody-drug conjugates (ADCs) and diagnostic reagents.

Application

• Bioconjugation and Protein Labeling: For the covalent attachment of fluorescent dyes, drugs, or other functional molecules to proteins, antibodies, or peptides via the NHS ester, with the azide handle allowing for subsequent click chemistry modifications.
• Antibody-Drug Conjugate (ADC) Development: Serves as a key linker component to connect cytotoxic payloads to monoclonal antibodies, leveraging the PEG spacer to improve solubility and pharmacokinetics.
• Surface Functionalization: Used to modify nanoparticles, biosensors, or microarray surfaces with azide groups for the immobilization of biomolecules through copper-catalyzed or strain-promoted azide-alkyne cycloaddition (SPAAC).
• Polymer and Material Science: Acts as a versatile building block for synthesizing functionalized hydrogels, smart polymers, and biomaterials with tailored biological activity.
• Diagnostic Reagent Synthesis: Employed in the production of enzyme conjugates, hapten-carrier protein complexes, and other reagents used in immunoassays (ELISA, lateral flow tests).
• Chemical Biology and Proteomics: Facilitates the study of protein-protein interactions, cellular trafficking, and post-translational modifications through bioorthogonal labeling strategies.

Basic Information

Product Name	Azido-PEG6-NHS Ester
CAS No.	2055014-64-5
Molecular Formula	C25H42N4O11
Molecular Weight	574.63 g/mol
Synonyms	NHS-PEG6-Azide; Azido-PEG6-NHS ester; 6-Azido-3,6,9,12,15,18-hexaoxahenicosan-1-oic acid 2,5-dioxopyrrolidin-1-yl ester; 1-Azido-3,6,9,12,15,18-hexaoxahenicosan-21-oic acid 2,5-dioxo-1-pyrrolidinyl ester; N-(Azido-PEG6)-N-hydroxysuccinimide ester; PEG6-Azide NHS Ester; Azidohexaethylene glycol NHS ester; N3-PEG6-NHS
EINECS	Contact for details

Quality Control

Our Azido-PEG6-NHS ester is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research and development applications. Each lot is characterized by HPLC, NMR, and mass spectrometry to confirm identity and purity. A comprehensive Certificate of Analysis (COA) detailing purity, functional group integrity, and absence of critical impurities is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This product is hygroscopic (moisture-sensitive) and the NHS ester moiety is susceptible to hydrolysis. For long-term storage, keep under an inert atmosphere. Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid or viscous oil
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Functional Group Assay (Azide)	≥ 90% of theoretical
Functional Group Assay (NHS Ester)	≥ 90% of theoretical

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.