Description

N3-Peg4-Cho is a heterobifunctional polyethylene glycol (PEG) crosslinker featuring an azide (N3) group and an aldehyde (CHO) group, separated by a tetraethylene glycol spacer. This compound is a critical reagent in bioconjugation and click chemistry, enabling the site-specific labeling and crosslinking of biomolecules under mild, aqueous conditions. It is essential for researchers and manufacturers in pharmaceutical development, proteomics, and advanced material science who require precise and stable linkages for drug delivery systems, diagnostic assays, and surface functionalization.

Application

• Bioconjugation and Antibody-Drug Conjugate (ADC) Development: Site-specific coupling of drugs, toxins, or fluorescent dyes to antibodies, proteins, or peptides via the aldehyde group for amine linkage and subsequent copper-free click chemistry with alkynes.
• Surface Functionalization and Hydrogel Formation: Modification of nanoparticles, chips, or polymer surfaces to introduce bio-recognition elements or create crosslinked networks for 3D cell culture and tissue engineering.
• Diagnostic Assay Development: Immobilization of capture antibodies or antigens onto solid supports for ELISA, lateral flow assays, and biosensors.
• Protein and Peptide Modification: Introduction of azide handles onto biomolecules for further functionalization via strain-promoted azide-alkyne cycloaddition (SPAAC).
• Polymer Chemistry: Synthesis of functionalized block copolymers and dendrimers with tailored properties for targeted delivery applications.
• Chemical Biology and Proteomics: As a tool compound for labeling and studying protein interactions, localization, and dynamics in live cells.

Basic Information

Product Name	N3-Peg4-Cho
CAS No.	2030118-14-8
Molecular Formula	C15H27N3O6
Molecular Weight	345.40 g/mol
Synonyms	Azido-PEG4-aldehyde; 1-Azido-3,6,9,12-tetraoxapentadecan-15-al; 15-Azido-4,7,10,13-tetraoxapentadecanal; N3-PEG4-CHO; Azido-PEG4-propionaldehyde; PEG4-azide-aldehyde crosslinker; Azido tetraethylene glycol aldehyde; Heterobifunctional PEG crosslinker, N3-PEG4-CHO
EINECS	Contact for details

Quality Control

Our N3-Peg4-Cho is manufactured under strict quality management systems. Every batch undergoes rigorous analytical testing to ensure high purity and consistent performance for sensitive bioconjugation applications. Certificates of Analysis (COA) detailing purity, identity, and functional group integrity are provided with each shipment. We support development under cGMP guidelines for pharmaceutical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a dry, moisture-free environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	Colorless to pale yellow viscous liquid or oil
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 0.5%
Functional Group Assay (NMR)	≥ 90% (Azide & Aldehyde)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.