Description

3’-Deoxy-3’-Fluoro-5’-o-(4,4’-Dimethoxytrityl)Thymidine is a crucial, protected nucleoside derivative used as a key synthetic intermediate in the production of oligonucleotides and modified nucleic acids. Its primary value lies in enabling the precise incorporation of a 3'-fluoro modification, which enhances the stability and biological activity of the resulting oligonucleotide sequences. This high-purity building block is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors developing advanced therapeutics, including antisense oligonucleotides and siRNA.

Application

• Oligonucleotide Synthesis: A critical phosphoramidite precursor for the solid-phase synthesis of modified DNA/RNA strands.
• Antisense Drug Development: Used to create nuclease-resistant oligonucleotides for therapeutic applications targeting genetic diseases.
• siRNA and miRNA Research: Incorporated to improve the metabolic stability and potency of RNA-based therapeutic agents.
• Diagnostic Probe Manufacture: Serves as a building block for labeled probes used in molecular diagnostics and hybridization assays.
• Structural Biology Studies: Employed to investigate nucleic acid structure, function, and protein interactions through modified nucleotides.
• Pharmaceutical Intermediates: A high-value intermediate in the multi-step synthesis of novel antiviral and anticancer nucleoside analogs.

Basic Information

Product Name	3’-Deoxy-3’-Fluoro-5’-o-(4,4’-Dimethoxytrityl)Thymidine
CAS No.	290371-78-7
Molecular Formula	C33H33FN2O7
Molecular Weight	588.63 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-3'-deoxy-3'-fluorothymidine; 3'-Fluoro-3'-deoxy-5'-O-DMT-thymidine; 3'-F-5'-O-DMT-Thd; DMT-3'-F-dT; 3'-Deoxy-3'-fluoro-5'-O-(4,4'-dimethoxytrityl)thymidine; 3'-Fluoro-5'-O-DMTr-thymidine; 3'-F-dT(DMT)
EINECS	Contact for details

Quality Control

Our 3’-Deoxy-3’-Fluoro-5’-o-(4,4’-Dimethoxytrityl)Thymidine is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity suitable for sensitive pharmaceutical applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to cGMP guidelines where applicable, and our quality protocols are designed to meet the stringent requirements of oligonucleotide synthesis and regulatory filings.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity. For long-term storage, consider storing under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Related Substances (HPLC)	Total impurities ≤ 2.0% Any single impurity ≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.