Description

N2-iso-Butyroyl-5'-o-(4,4'-Dimethoxytrityl)-3'-o-(Methoxyethyl)Guanosine CAS NO 256224-05-2 is a high-purity, protected nucleoside phosphoramidite building block, specifically designed for solid-phase oligonucleotide synthesis. This compound is critical for ensuring high coupling efficiency and sequence fidelity in the automated production of DNA and RNA strands. It is an essential raw material for research institutions, diagnostic developers, and pharmaceutical companies engaged in the development of antisense oligonucleotides, siRNAs, aptamers, and other advanced therapeutic modalities.

Application

• Solid-Phase Oligonucleotide Synthesis: Serves as a key monomer (Guanosine derivative) in automated synthesizers for the stepwise construction of custom DNA and RNA sequences.
• Therapeutic Oligonucleotide Manufacturing: Used in the GMP-compliant production of active pharmaceutical ingredients (APIs) for antisense, siRNA, and miRNA-based therapies.
• Diagnostic Probe Development: Essential for synthesizing labeled or modified probes used in PCR, FISH, microarrays, and next-generation sequencing applications.
• Research-Grade Oligo Production: Enables the synthesis of high-purity oligonucleotides for basic biochemical and molecular biology research.
• Aptamer and SELEX Library Construction: Provides the modified nucleoside foundation for creating libraries of nucleic acid aptamers with enhanced stability or binding properties.
• Synthesis of Modified RNAs: Facilitates the incorporation of site-specific modifications into RNA strands to study structure-function relationships or enhance therapeutic profiles.

Basic Information

Product Name	N2-iso-Butyroyl-5'-o-(4,4'-Dimethoxytrityl)-3'-o-(Methoxyethyl)Guanosine
CAS No.	256224-05-2
Molecular Formula	C41H47N5O9
Molecular Weight	753.85 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-deoxy-2'-(2-methoxyethoxy)-N2-isobutyrylguanosine; 5'-O-DMT-2'-O-MOE-N2-iso-butyryl-Guanosine; DMT-G(iBu)-2'-O-MOE-CE Phosphoramidite precursor; 2'-O-(2-Methoxyethyl)-N2-isobutyryl-5'-O-(4,4'-dimethoxytrityl)guanosine; Guanosine, 5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2'-O-(2-methoxyethyl)-N2-(2-methylpropanoyl)-; Protected MOE-Guanosine monomer; RNA Phosphoramidite Building Block (G)
EINECS	Contact for details

Quality Control

Every batch of this protected nucleoside is manufactured under strict quality management systems. We employ advanced analytical techniques, including HPLC, NMR, and mass spectrometry, to ensure identity, purity, and consistency meet the exacting standards required for oligonucleotide synthesis. A comprehensive Certificate of Analysis (COA) detailing purity (typically ≥98.5% by HPLC), moisture content, and spectroscopic data is provided with each shipment to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C ± 5°C under an inert atmosphere (argon or nitrogen) in a dry, freezer. This product is hygroscopic (moisture-sensitive). Allow the sealed container to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.5%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in acetonitrile, DCM, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.