Description

3'-o-Moe-A(Bz)-2'-Ced-Phosphoramidite is a high-purity, protected phosphoramidite monomer essential for the solid-phase synthesis of oligonucleotides. This compound matters because it enables the precise and efficient incorporation of modified adenosine residues, which are critical for enhancing the stability and therapeutic efficacy of synthetic RNA and DNA strands. It is primarily needed by pharmaceutical R&D teams, biotechnology companies, and CROs engaged in the development of antisense oligonucleotides, siRNA, aptamers, and other advanced nucleic acid therapeutics.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key building block (monomer) in automated DNA/RNA synthesizers for the stepwise assembly of oligonucleotide chains.
• Antisense Oligonucleotide (ASO) Development: For creating therapeutic ASOs with improved nuclease resistance and binding affinity through 2'-O-methoxyethyl (MOE) and 5'-O-benzoyl (Bz) modifications.
• siRNA and miRNA Synthesis: Used in the production of small interfering RNA (siRNA) and microRNA (miRNA) analogs for gene silencing research and therapeutic applications.
• Aptamer and Diagnostic Probe Manufacturing: Essential for synthesizing high-affinity nucleic acid aptamers and labeled probes used in diagnostics and biosensors.
• Research Scale Synthesis: For academic and industrial research into novel oligonucleotide sequences, modifications, and structure-activity relationships (SAR).
• Scale-Up and GMP Production: Suitable for process development and current Good Manufacturing Practice (cGMP) production of oligonucleotide active pharmaceutical ingredients (APIs).

Basic Information

Product Name	3'-o-Moe-A(Bz)-2'-Ced-Phosphoramidite
CAS No.	256224-01-8
Molecular Formula	C43H53N6O8P
Molecular Weight	812.90 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-O-methoxyethyl-N6-benzoyladenosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 2'-O-MOE-5'-O-DMT-N6-Bz-Adenosine-3'-CE Phosphoramidite; 2'-O-(2-Methoxyethyl)-5'-O-DMT-N6-Bz-A-3'-phosphoramidite; MOE-A(Bz) Phosphoramidite; 2'-MOE Adenosine Phosphoramidite (Bz protected); DMT-2'-O-MOE-ABz-CE Phosphoramidite; 5'-DMT-2'-O-MOE-N6-Bz-adenosine-3'-CE phosphoramidite
EINECS	Contact for details

Quality Control

Every batch of 3'-o-Moe-A(Bz)-2'-Ced-Phosphoramidite is manufactured and tested under strict quality management systems to ensure consistency and reliability for critical oligonucleotide synthesis. Our products undergo rigorous quality testing, including HPLC purity analysis, NMR structural confirmation, and 31P NMR for phosphorous assay, to ensure compliance with industry standards. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Assay (31P NMR)	≥ 97.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.