Description

Adenosine, 5'-o-[Bis(4-Methoxyphenyl)Phenylmethyl]-2'-Fluoro-2'-Deoxy-, 3'-[2-Cyanoethyl Bis(1-Methylethyl)Phosphoramidite] is a high-purity phosphoramidite monomer, a critical building block for the solid-phase synthesis of oligonucleotides. This compound is specifically engineered to introduce a 2'-fluoro modification into RNA sequences, significantly enhancing their nuclease resistance and thermal stability for advanced therapeutic and diagnostic applications. It is an essential raw material for research institutions and pharmaceutical companies developing antisense oligonucleotides, siRNA, aptamers, and other next-generation nucleic acid-based drugs.

Application

• Synthesis of 2'-Fluoro-modified Antisense Oligonucleotides (ASOs): For therapeutic applications targeting genetic diseases, where the fluorine substitution improves metabolic stability and binding affinity.
• Production of Stabilized Small Interfering RNA (siRNA): Used in RNA interference (RNAi) therapeutics to enhance duplex stability and efficacy by reducing degradation.
• Aptamer Development: For creating nuclease-resistant aptamers used in diagnostics, biosensors, and targeted drug delivery systems.
• Research-Grade Oligonucleotide Synthesis: For academic and industrial R&D focused on studying RNA structure, function, and protein-RNA interactions.
• Diagnostic Probe Manufacture: In the production of fluorescently labeled probes and primers for qPCR, FISH, and other molecular diagnostic assays requiring high specificity and stability.
• Synthesis of Spiegelmers and other Chiral Oligonucleotides: For developing enantiomerically pure therapeutic agents.

Basic Information

Product Name	Adenosine, 5'-o-[Bis(4-Methoxyphenyl)Phenylmethyl]-2'-Fluoro-2'-Deoxy-, 3'-[2-Cyanoethyl Bis(1-Methylethyl)Phosphoramidite]
CAS No.	252770-65-3
Molecular Formula	C43H51FN6O7P
Molecular Weight	813.89 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-2'-fluoro-2'-deoxyadenosine-3'-[(2-cyanoethyl)-N,N-diisopropylphosphoramidite]; 2'-F-dA Phosphoramidite; 2'-Fluoro-2'-deoxyadenosine 3'-CE Phosphoramidite; DMT-2'-F-dA-CE Phosphoramidite; 2'-F-Adenosine Phosphoramidite; 5'-DMT-2'-fluoro-2'-deoxyadenosine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 2'-F-dA-CEP
EINECS	Contact for details

Quality Control

This high-value nucleic acid building block is manufactured under strict quality control protocols in a dedicated synthesis environment. Each batch is characterized using advanced analytical techniques including HPLC, 31P NMR, and 1H NMR to ensure identity, purity, and suitability for automated oligonucleotide synthesis. Certificates of Analysis (COA) with detailed chromatographic data are provided for every lot, supporting compliance with internal specifications for pharmaceutical R&D.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and easily oxidized. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation. For long-term stability, store in the original sealed packaging.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Moisture Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.