Description

Methyl 4-Nitrophenyl Phosphate is a specialized organophosphate ester compound, serving as a key biochemical reagent and synthetic intermediate. Its primary commercial value lies in its role as a chromogenic substrate for the detection and quantification of alkaline phosphatase activity in diagnostic and research applications. This product is essential for manufacturers and research institutions in the pharmaceutical, diagnostic kit production, and life science research sectors, where precise enzymatic analysis is critical.

Application

• Diagnostic Reagent: Primary substrate for colorimetric assays measuring alkaline phosphatase (ALP) activity in clinical diagnostics and ELISA kits.
• Biochemical Research: Used in enzymatic studies, inhibitor screening, and kinetic analysis in academic and industrial R&D laboratories.
• Pharmaceutical Development: Employed in drug discovery processes for high-throughput screening (HTS) assays targeting phosphatase enzymes.
• Molecular Biology: Utilized in nucleic acid detection systems and blotting techniques that employ enzyme-conjugated probes.
• Chemical Synthesis: Acts as a phosphorylating agent or protected phosphate intermediate in organic synthesis.
• Quality Control: Serves as a reference standard for calibrating diagnostic equipment and validating assay performance.

Basic Information

Product Name	Methyl 4-Nitrophenyl Phosphate
CAS No.	15930-83-3
Molecular Formula	C7H8NO6P
Molecular Weight	233.12 g/mol
Synonyms	4-Nitrophenyl Methyl Phosphate; p-Nitrophenyl Methyl Phosphate; Methyl p-Nitrophenyl Phosphate; PNMP; Phosphoric Acid, Methyl 4-Nitrophenyl Ester; 4-Nitrophenyl Phosphate Monomethyl Ester; Methyl Ester of 4-Nitrophenyl Phosphoric Acid
EINECS	Contact for details

Quality Control

Our Methyl 4-Nitrophenyl Phosphate is manufactured under a strict quality management system to ensure batch-to-batch consistency and high purity suitable for sensitive diagnostic and research applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. A Certificate of Analysis (COA) detailing specific results for assay, impurities, and physical properties is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). The product is light-sensitive and should be handled under appropriate conditions to prevent degradation. Keep the container sealed to minimize exposure to atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content	≤ 1.0%
Solubility	Soluble in water, buffers, and polar organic solvents
Heavy Metals	< 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.