Description

5-[n-(6-Aminohexyl)-3-(E)-Acrylamido]-5'-o-(Dimethoxytrityl)-2'-Deoxyuridine is a sophisticated, high-purity nucleoside phosphoramidite precursor, specifically engineered for oligonucleotide synthesis. This compound is critical for introducing functional handles or labels into synthetic DNA strands, enabling advanced applications in diagnostics and therapeutics. It is an essential raw material for research institutions and manufacturers developing next-generation antisense oligonucleotides, aptamers, and probes for molecular biology and pharmaceutical R&D.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite monomer for the solid-phase synthesis of modified DNA strands.
• Functionalized Probe Development: Enables the site-specific incorporation of reactive aminoalkylacrylamide groups for post-synthetic conjugation with fluorophores, quenchers, or other reporter molecules.
• Therapeutic Nucleic Acid Research: Used in the development of antisense oligonucleotides (ASOs) and siRNA constructs that require precise chemical modification for stability and targeting.
• Diagnostic Assay Manufacturing: Essential for creating labeled probes used in PCR, FISH, microarray, and other hybridization-based diagnostic platforms.
• Aptamer Selection (SELEX): Provides a versatile scaffold for introducing diversity and functionality into synthetic nucleic acid libraries.
• Bioconjugation and Immobilization: The reactive acrylamido group allows for covalent attachment of oligonucleotides to surfaces, polymers, or proteins.

Basic Information

Item	Details
Product Name	5-[n-(6-Aminohexyl)-3-(E)-Acrylamido]-5'-o-(Dimethoxytrityl)-2'-Deoxyuridine
CAS No.	252337-60-3
Molecular Formula	C41H49N5O8
Molecular Weight	739.87 g/mol
Synonyms	5-[N-(6-Aminohexyl)-3-acrylamido]-2'-deoxyuridine, 5'-O-(4,4'-Dimethoxytrityl)-; 5-(N-(6-Aminohexyl)acrylamido)-2'-deoxyuridine 5'-O-DMT; Amino-Modifier C6 dT Phosphoramidite precursor; 5-[3-(6-Aminohexyl)ureido]-2'-deoxyuridine DMT derivative (related); DMT-dU-C6-Amino-Acrylamide; 5-(3-Acrylamidopropyl)uridine derivative; 2'-Deoxy-5-[N-(6-aminohexyl)-3-acrylamido]uridine 5'-O-DMT
EINECS	Contact for details

Quality Control

Every batch of our 5-[n-(6-Aminohexyl)-3-(E)-Acrylamido]-5'-o-(Dimethoxytrityl)-2'-Deoxyuridine is manufactured under strict quality management systems and undergoes comprehensive analytical characterization. We ensure compliance with the high-purity standards required for sensitive oligonucleotide synthesis. A detailed Certificate of Analysis (COA) providing HPLC purity, identity confirmation (NMR, MS), and moisture content is supplied with each shipment to guarantee batch-to-batch consistency and traceability.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, freezer. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.