Description

5'-O-Dmt-N2-Isobutyryl-2'-O-(2-Methoxyethyl)Guanosine is a key phosphoramidite monomer used in the solid-phase synthesis of oligonucleotides. This chemically modified nucleoside is critical for producing high-quality, nuclease-resistant RNA analogs, such as those used in therapeutic antisense and siRNA applications. It is an essential raw material for pharmaceutical R&D, diagnostic kit manufacturers, and biotechnology companies engaged in advanced nucleic acid research.

Application

• Therapeutic Oligonucleotide Synthesis: Primary building block for manufacturing antisense oligonucleotides (ASOs) and small interfering RNA (siRNA) with 2'-MOE (2'-O-methoxyethyl) modifications.
• Research & Development: Used in academic and industrial labs for the study of RNA interference (RNAi) mechanisms and the development of novel gene-silencing therapeutics.
• Diagnostic Probe Production: Enables the synthesis of stable, high-affinity nucleic acid probes for molecular diagnostics and in-situ hybridization assays.
• Synthesis of Aptamers: Facilitates the creation of nuclease-resistant RNA aptamers for targeted drug delivery and biomarker detection.
• GMP Manufacturing: Serves as a critical starting material in cGMP production environments for clinical-grade oligonucleotide APIs.

Basic Information

Product Name	5'-O-Dmt-N2-Isobutyryl-2'-O-(2-Methoxyethyl)Guanosine
CAS No.	251647-50-4
Molecular Formula	C38H45N5O9
Molecular Weight	715.80 g/mol
Synonyms	2'-O-MOE-Guanosine DMT Derivative; N2-Isobutyryl-5'-O-(4,4'-dimethoxytrityl)-2'-O-(2-methoxyethyl)guanosine; 5'-O-DMT-2'-O-MOE-G(Ibu) Amidite Precursor; Guanosine, 2'-O-(2-methoxyethyl)-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-N2-(2-methyl-1-oxopropyl)-; 2'-MOE-Guanosine Phosphoramidite Monomer (protected); DMT-2'-MOE-G(Ibu)-OH
EINECS	Contact for details

Quality Control

Every batch of 5'-O-Dmt-N2-Isobutyryl-2'-O-(2-Methoxyethyl)Guanosine is manufactured and tested under strict quality systems. We ensure compliance with stringent internal specifications designed for nucleic acid synthesis applications. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles by HPLC and NMR is provided with each shipment to guarantee reproducible performance in your oligonucleotide assembly processes.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere to maintain stability. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.