Description

[5'-13C]Thymidine is a stable isotope-labeled nucleoside, specifically labeled with carbon-13 at the 5' position of the deoxyribose sugar. This compound is essential for advanced research in life sciences, providing a critical tool for metabolic pathway tracing and quantitative analysis. It is primarily utilized by pharmaceutical R&D teams, academic research institutions, and diagnostic developers working in drug metabolism studies (DMPK), biomarker discovery, and nucleic acid research.

Application

• Drug Metabolism and Pharmacokinetics (DMPK) Studies: Used as an internal standard in LC-MS/MS for the accurate quantification of endogenous thymidine and its metabolites in biological matrices.
• Nucleic Acid Metabolism Research: Enables tracing of DNA synthesis and repair pathways in cell culture and in vivo models.
• Biomarker Discovery & Validation: Facilitates the development of stable isotope dilution mass spectrometry (SID-MS) assays for clinical diagnostics.
• Cancer and Cell Proliferation Research: Employed in studies investigating nucleotide salvage pathways and the efficacy of chemotherapeutic agents.
• Virology and Microbiology: Used to study viral DNA replication and the metabolism of microbial organisms.
• Synthesis of Labeled Compounds: Serves as a key building block for the synthesis of more complex carbon-13 labeled nucleotides and oligonucleotides.

Basic Information

Product Name	[5'-13C]Thymidine
CAS No.	240407-53-8
Molecular Formula	C10H1413CN2O5
Molecular Weight	Contact for details
Synonyms	1-(2-Deoxy-5-O-phosphono-β-D-erythro-pentofuranosyl)-5-methyl-2,4(1H,3H)-pyrimidinedione 5'-13C; Thymidine-5'-13C; 2'-Deoxythymidine-5'-13C; dT-5'-13C; 1-[(2R,4S,5R)-4-Hydroxy-5-(hydroxymethyl13C)oxolan-2-yl]-5-methylpyrimidine-2,4-dione
EINECS	Contact for details

Quality Control

Our [5'-13C]Thymidine is manufactured under strict quality control protocols to ensure high chemical purity and isotopic enrichment. Each batch is analyzed to confirm identity, purity, and isotopic abundance, with comprehensive Certificates of Analysis (COA) provided. We support research requiring compliance with cGMP and ISO 9001 standards for traceable reference materials.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry, cool place. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider storing desiccated under inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥98%
Isotopic Enrichment (13C)	≥99 atom % 13C
Chemical Purity (NMR)	Conforms
Water Content (KF)	≤1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.