Description

Cytidine, n-Benzoyl-2'-O,4'-C-Methylene- (9Ci) is a chemically modified nucleoside derivative, specifically engineered for advanced oligonucleotide synthesis. This compound matters as a critical building block in the development of antisense oligonucleotides and siRNA therapeutics, where its locked nucleic acid (LNA)-like structure enhances binding affinity and stability. It is primarily needed by pharmaceutical R&D teams, biotechnology companies, and CROs specializing in nucleic acid chemistry and next-generation therapeutic modalities.

Application

• Pharmaceutical Intermediate: A key monomer in the solid-phase synthesis of therapeutic oligonucleotides, including antisense drugs and siRNA.
• Research & Development: Used in academic and industrial labs for studying nucleic acid structure, function, and for developing novel diagnostic probes.
• Bioconjugation: Serves as a modified cytidine base for creating labeled or tagged oligonucleotides for imaging and targeting applications.
• Antiviral & Anticancer Agent Development: Investigated as a component in nucleotide analogs designed to inhibit viral replication or cancer cell proliferation.
• Diagnostic Probe Synthesis: Employed in the manufacture of high-specificity probes for genetic testing and molecular diagnostics.

Basic Information

Product Name	Cytidine, n-Benzoyl-2'-O,4'-C-Methylene- (9Ci)
CAS No.	221229-74-9
Molecular Formula	C17H17N3O5
Molecular Weight	343.34 g/mol
Synonyms	4-C-Methylene-2'-O-methylenecytidine, N4-Benzoyl derivative; 2'-O,4'-C-Methylenecytidine N4-benzoyl; Bz-C-Methylene-Cytidine; LNA-Cytidine(Bz) phosphoramidite precursor; 1-(4-C,2-O-Dimethylene-β-D-ribofuranosyl)-4-benzamidopyrimidin-2-one; N4-Benzoyl-2'-O,4'-C-methylenecytidine; 9CI Listed Cytidine Derivative; 2'-O,4'-C-Methylenecytidine, N4-benzoyl-
EINECS	Contact for details

Quality Control

Our production of this high-purity biochemical intermediate adheres to stringent quality protocols. Every batch is characterized and tested using advanced analytical techniques including HPLC, NMR, and mass spectrometry to ensure identity, purity, and consistency. Certificates of Analysis (COA) documenting full specifications are provided and we support compliance with relevant GMP and ICH guidelines for pharmaceutical applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere to maintain stability. Keep the container tightly sealed when not in use.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH limits

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.