Description

5'-O-(Dimethoxytrityl)-N2-Ethyl-2'-Deoxyguanosine is a high-purity, protected nucleoside derivative essential for oligonucleotide synthesis. This compound serves as a critical phosphoramidite building block, enabling the precise and automated assembly of DNA sequences for research and therapeutic applications. It is primarily required by biotechnology and pharmaceutical companies engaged in antisense drug development, PCR primer synthesis, and genetic research.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated synthesis of DNA strands.
• Antisense Oligonucleotide (ASO) Development: For creating therapeutic nucleic acids that target specific RNA sequences.
• Research-Grade Primer and Probe Synthesis: Manufacturing custom primers for PCR, qPCR, and other molecular biology techniques.
• Synthesis of Modified DNA: Incorporating site-specific ethyl modifications into oligonucleotides to alter properties like stability or binding affinity.
• Bioconjugation and Labeling: Serving as an intermediate for attaching reporter molecules or functional groups to DNA.
• Academic and Industrial R&D: For fundamental studies in genomics, molecular biology, and nucleic acid chemistry.

Basic Information

Product Name	5'-O-(Dimethoxytrityl)-N2-Ethyl-2'-Deoxyguanosine
CAS No.	209785-64-8
Molecular Formula	C33H35N5O6
Molecular Weight	597.67 g/mol
Synonyms	5'-O-DMT-N2-Ethyl-2'-deoxyguanosine; N2-Ethyl-5'-O-(4,4'-dimethoxytrityl)-2'-deoxyguanosine; DMT-dG(Et) (protected nucleoside); 2'-Deoxy-N2-ethyl-5'-O-(4,4'-dimethoxytrityl)guanosine; N2-Ethyl-2'-deoxyguanosine DMT ether; Guanosine, 2'-deoxy-N2-ethyl-, 5'-(4,4'-dimethoxytrityl) ether
EINECS	Contact for details

Quality Control

Our 5'-O-(Dimethoxytrityl)-N2-Ethyl-2'-Deoxyguanosine is manufactured under strict quality systems to ensure it meets the exacting standards required for oligonucleotide synthesis. Each batch is subjected to rigorous analytical testing, including HPLC, NMR, and mass spectrometry, to guarantee identity, purity, and consistency. Certificates of Analysis (COA) with full analytical data are provided for every lot to support your quality assurance and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in acetonitrile, DMF, pyridine

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.