Description

N6-(Diisobutylaminomethylidene)-5'-o- (Dimethoxytrityl)-O2-(Diphenylcarbamoyl)-2'- Deoxyisoguanosine is a sophisticated, high-purity nucleoside phosphoramidite derivative, a critical building block in the automated synthesis of oligonucleotides. Its primary value lies in enabling the precise and efficient incorporation of modified bases into synthetic DNA and RNA strands, which is essential for advanced life science research and therapeutic development. This compound is specifically designed for use by researchers and manufacturers in the fields of antisense therapy, diagnostic probe development, and synthetic biology, where sequence-specific functionality is paramount.

Application

• Oligonucleotide Synthesis: As a phosphoramidite monomer for the solid-phase synthesis of modified DNA strands, particularly for introducing isoguanosine analogs.
• Antisense and siRNA Therapeutics: A key raw material for creating research-grade and therapeutic oligonucleotides designed for gene silencing and targeted modulation of gene expression.
• Diagnostic Probe Manufacturing: Used in the production of high-specificity probes for fluorescence in situ hybridization (FISH), PCR, and next-generation sequencing applications.
• Aptamer Development: Enables the creation of synthetic nucleic acid libraries (SELEX) for discovering aptamers with high binding affinity to specific targets.
• Structural Biology Studies: Incorporation into oligonucleotides to study nucleic acid structure, stability, and protein-nucleic acid interactions.
• Synthetic Biology and Xenobiology: A component for expanding the genetic alphabet and creating artificial nucleic acid systems with novel properties.

Basic Information

Product Name	N6-(Diisobutylaminomethylidene)-5'-o- (Dimethoxytrityl)-O2-(Diphenylcarbamoyl)-2'- Deoxyisoguanosine
CAS No.	207981-37-1
Molecular Formula	C52H61N7O7
Molecular Weight	896.10 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N6-(diisobutylaminomethylene)-2'-deoxyisoguanosine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 2'-Deoxyisoguanosine, N6-[(diisobutylamino)methylene]-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-O2-(diphenylcarbamoyl)-, 3'-[2-cyanoethyl bis(1-methylethyl)phosphoramidite]; dIsoG Phosphoramidite; Iso-dG Phosphoramidite; 2'-Deoxyisoguanosine Phosphoramidite (DMT, iBu).
EINECS	Contact for details

Quality Control

Our production of this high-value phosphoramidite adheres to stringent quality protocols suitable for research and pharmaceutical-grade applications. Every batch is characterized and tested to ensure identity, purity, and performance in oligonucleotide synthesis. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing key parameters such as purity (HPLC), moisture content (Karl Fischer), and identity confirmation (NMR, MS). We operate under a quality management system to ensure consistency, traceability, and reliability for your critical projects.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions, moisture, or light will lead to degradation and reduced coupling efficiency. Allow the sealed vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.