Description

8-Bromo-5'-O-(4,4'-Dimethoxytrityl)-N6-Dimethylaminomethylene-2'-Deoxyadenosine, 3'-[(2-Cyanoethyl)-(N,N-Diisopropyl)]Phosphoramidite is a high-purity, protected phosphoramidite monomer essential for the solid-phase synthesis of oligonucleotides. This compound is specifically designed for the controlled incorporation of modified adenine bases, enabling the creation of advanced DNA and RNA sequences for research and therapeutic applications. It is a critical raw material for biotechnology, pharmaceutical R&D, and diagnostic kit manufacturers focused on nucleic acid synthesis.

Application

• Oligonucleotide Synthesis: As a key phosphoramidite building block for automated solid-phase DNA/RNA synthesizers.
• Antisense & siRNA Therapeutics: For the production of modified oligonucleotides used in gene silencing and therapeutic applications.
• PCR Primers & Probes: Enables the synthesis of primers and fluorescent probes with specific base modifications for enhanced diagnostic assays.
• Structural Biology Studies: Used to incorporate brominated nucleosides for phasing in X-ray crystallography.
• Aptamer Development: Critical for creating modified DNA/RNA libraries in SELEX processes to develop high-affinity binding molecules.
• Next-Generation Sequencing (NGS): Used in the synthesis of specialized adapters or primers for novel sequencing platforms.

Basic Information

Product Name	8-Bromo-5'-O-(4,4'-Dimethoxytrityl)-N6-Dimethylaminomethylene-2'-Deoxyadenosine, 3'-[(2-Cyanoethyl)-(N,N-Diisopropyl)]Phosphoramidite
CAS No.	207906-54-5
Molecular Formula	C48H55BrN7O7P
Molecular Weight	953.88 g/mol
Synonyms	8-Br-dA Phosphoramidite (DMTr protected); 8-Bromo-2'-deoxyadenosine phosphoramidite; 5'-O-(4,4'-Dimethoxytrityl)-8-bromo-2'-deoxyadenosine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]phosphoramidite; N6-Dimethylaminomethylene-8-bromo-2'-deoxyadenosine phosphoramidite; 8-Bromo-dA-CE Phosphoramidite; 207906-54-5; Amidite for 8-Bromo-deoxyadenosine
EINECS	Contact for details

Quality Control

Our 8-Bromo-5'-O-(4,4'-Dimethoxytrityl)-N6-Dimethylaminomethylene-2'-Deoxyadenosine, 3'-[(2-Cyanoethyl)-(N,N-Diisopropyl)]Phosphoramidite is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC, 31P NMR, and 1H NMR, to ensure identity, purity, and suitability for oligonucleotide synthesis. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment to guarantee traceability and batch-to-batch consistency for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This compound is strictly light-sensitive and hygroscopic. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (31P NMR)	Conforms to reference standard
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.