Description

2'-O,4'-C-Methyleneguanosine is a chemically modified nucleoside analog, a key building block in advanced oligonucleotide synthesis. This high-purity intermediate is critical for the development and manufacturing of next-generation therapeutic nucleic acids, including antisense oligonucleotides and small interfering RNAs (siRNAs). It is primarily utilized by pharmaceutical R&D teams, biotechnology companies, and CDMOs focused on creating novel genetic medicines with enhanced stability and specificity.

Application

• Oligonucleotide Synthesis: A vital phosphoramidite precursor for the solid-phase synthesis of therapeutic oligonucleotides.
• Antisense Drug Development: Used to incorporate locked nucleic acid (LNA) or bridged nucleic acid (BNA) motifs, enhancing target binding affinity and metabolic stability.
• siRNA Therapeutics: Enables the production of chemically modified siRNA strands to improve potency and reduce off-target effects.
• Research & Diagnostic Probes: Serves in the creation of high-specificity probes for genetic testing, FISH, and other molecular biology applications.
• Apiamer Development: Employed in the synthesis of modified aptamers for therapeutic and diagnostic use.
• Process Development & Scale-up: A critical raw material for process chemistry teams scaling up GMP manufacturing of oligonucleotide APIs.

Basic Information

Product Name	2'-O,4'-C-Methyleneguanosine
CAS No.	207131-16-6
Molecular Formula	C12H15N5O5
Molecular Weight	309.28 g/mol
Synonyms	2'-O,4'-C-Methylene Guanosine; 2'-O,4'-C-Methyleneguanosine; BNA-Guanosine; LNA-Guanosine (bridged form); 9-[(1S,3R,4R,7S)-7-Hydroxy-2,5-dioxa-4-(phosphonooxy)bicyclo[2.2.1]hept-1-yl]-1,9-dihydro-6H-purin-6-one (related to phosphate); Guanosine, 2'-O,4'-C-methylene-; 2'-O,4'-C-Methyleneguanosine (BNA monomer); 207131-16-6
EINECS	Contact for details

Quality Control

Our 2'-O,4'-C-Methyleneguanosine is manufactured under strict quality systems to ensure it meets the exacting standards required for pharmaceutical development. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We support compliance with ICH Q7 guidelines and can provide material suitable for use in GMP manufacturing upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled under a dry atmosphere or inert gas to maintain stability. For long-term storage, consider desiccated conditions at -20°C.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to reference spectrum
Assay (HPLC)	≥ 98.0%
Purity (HPLC, Area%)	≥ 99.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH Q3C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.