Description

2'-O,4'-C-Methylenecytidine is a chemically modified nucleoside analog, specifically a bridged nucleic acid (BNA) derivative. This structural modification confers enhanced binding affinity and nuclease resistance, making it a critical building block for advanced oligonucleotide therapeutics and research. It is essential for pharmaceutical R&D, diagnostic assay development, and academic research in molecular biology and antisense technology.

Application

• Oligonucleotide Synthesis: A key monomer for constructing antisense oligonucleotides (ASOs), siRNA, and aptamers with improved stability and target affinity.
• Pharmaceutical Research: Used in the development of novel therapeutic agents for genetic disorders, oncology, and antiviral applications.
• Diagnostic Probes: Incorporated into high-sensitivity probes for PCR, FISH, and other molecular diagnostic techniques.
• Biochemical Research: Serves as a tool for studying RNA structure, function, and protein-nucleic acid interactions.
• Academic & Contract Research: Fundamental reagent for universities and CROs exploring nucleic acid chemistry and drug discovery.

Basic Information

Product Name	2'-O,4'-C-Methylenecytidine
CAS No.	206055-69-8
Molecular Formula	C10H13N3O5
Molecular Weight	255.23 g/mol
Synonyms	2'-O,4'-C-Methylenecytidine; 2'-O,4'-C-Methylene Cytidine; LNA-Cytidine monomer; BNA-Cytidine; 4'-C-(Hydroxymethyl)-2'-O-methylcytidine; 1-[(2R,3R,4R,5R)-5-(Hydroxymethyl)-3,4-dihydroxy-4-(hydroxymethyl)oxolan-2-yl]pyrimidine-2,4-dione; Locked Nucleic Acid Cytidine; 2'-O,4'-C-Methylenecitidine
EINECS	Contact for details

Quality Control

Our 2'-O,4'-C-Methylenecytidine is manufactured under strict quality management systems. Each batch is subjected to comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and tests for residual solvents and moisture. We provide full traceability and Certificates of Analysis (COA) are available for all shipments to ensure compliance with your research and development requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and should be handled under dry conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.