Description

D4 Pregnane CAS NO 205529-77-7 is a high-purity, deuterium-labeled steroid derivative, specifically a pregnane molecule where four hydrogen atoms have been replaced with deuterium. This stable isotope labeling is critical for advanced analytical applications, providing a non-radioactive tracer with distinct mass spectral properties. It is primarily utilized by research institutions and pharmaceutical companies engaged in metabolic studies, pharmacokinetic research, and as an internal standard for quantitative mass spectrometry.

Application

• Internal Standard for LC-MS/MS: Serves as a crucial, isotopically-labeled internal standard for the precise quantification of endogenous pregnane steroids and related metabolites in complex biological matrices.
• Metabolic Pathway Tracing: Used in deuterium tracing studies to investigate steroidogenesis, metabolic flux, and the biotransformation pathways of pregnane-based compounds.
• Pharmaceutical Reference Standard: Employed as a certified reference material (CRM) in drug development for method validation, quality control, and ensuring regulatory compliance for steroid-based therapeutics.
• Biochemical Research: Facilitates fundamental research into steroid hormone receptors, enzyme mechanisms (e.g., cytochrome P450), and endocrine system function.
• Doping Control Analysis: Acts as a reference compound in sports anti-doping laboratories for the accurate detection and confirmation of synthetic steroid analogs.

Basic Information

Product Name	D4 Pregnane
CAS No.	205529-77-7
Molecular Formula	C21H32D4
Molecular Weight	~292.53 g/mol
Synonyms	5β-Pregnane-2,2,4,4-d4; (5β)-Pregnane-2,2,4,4-d4; 2,2,4,4-Tetradeuterio-5β-pregnane; Deuterated Pregnane; Pregnane-d4; Tetradeuterated Pregnane; 5β-Pregnane-d4; NSC 151881-d4
EINECS	Contact for details

Quality Control

Our D4 Pregnane is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including NMR and mass spectrometry, to confirm isotopic enrichment and chemical purity. We provide a detailed Certificate of Analysis (COA) with each shipment, which includes batch-specific data on purity, isotopic purity (D-enrichment), and residual solvent analysis. Our quality commitment ensures the material meets the stringent requirements for use as a research and diagnostic reference standard.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to minimize exposure to atmospheric humidity. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Isotopic Purity (D-enrichment)	≥ 98 atom % D
Water Content (KF)	≤ 0.5%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.