Description

8-Bromo-5'-o-(Dimethoxytrityl)-N2-(Dimethylaminomethylidene)-2'-Deoxyguanosine is a high-purity, protected nucleoside phosphoramidite precursor, a critical building block in the automated synthesis of oligonucleotides. This compound is engineered for precise and efficient incorporation into DNA sequences, enabling the development of advanced research tools and therapeutic agents. It is essential for pharmaceutical R&D, diagnostic assay development, and academic research focused on genomics, antisense technology, and siRNA applications.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated synthesis of modified DNA strands.
• Therapeutic Oligonucleotide Development: For creating antisense oligonucleotides (ASOs), siRNA, and aptamers with enhanced stability and targeting properties.
• Diagnostic Probe Manufacture: Used in the production of labeled probes for PCR, FISH, and microarray technologies.
• Basic Research: A vital reagent in academic and industrial labs studying nucleic acid chemistry, structure, and function.
• Bioconjugation: The reactive bromo group allows for site-specific labeling and conjugation with various reporter molecules or solid supports.
• Scale-Up GMP Production: Suitable for the cGMP-compliant manufacture of oligonucleotide-based active pharmaceutical ingredients (APIs).

Basic Information

Product Name	8-Bromo-5'-o-(Dimethoxytrityl)-N2-(Dimethylaminomethylidene)-2'-Deoxyguanosine
CAS No.	204582-53-6
Molecular Formula	C34H35BrN6O6
Molecular Weight	695.59 g/mol
Synonyms	8-Bromo-dG(DMT) Amidite; 8-Br-dG Phosphoramidite; 5'-O-(4,4'-Dimethoxytrityl)-8-bromo-2'-deoxyguanosine, 3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; N2-(Dimethylaminomethylidene)-5'-O-(4,4'-dimethoxytrityl)-8-bromo-2'-deoxyguanosine; 8-Bromo-2'-deoxyguanosine derivative; DMTr-8-Br-dG-amidite; Guanosine, 2'-deoxy-8-bromo-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-2-(dimethylaminomethylene)amino]-
EINECS	Contact for details

Quality Control

Every batch of 8-Bromo-5'-o-(Dimethoxytrityl)-N2-(Dimethylaminomethylidene)-2'-Deoxyguanosine is manufactured and tested under strict quality management systems. We provide comprehensive Certificates of Analysis (COA) detailing purity, identity, and moisture content, ensuring compliance with the stringent requirements for oligonucleotide synthesis. Our quality protocols are designed to support research-grade to cGMP applications, with traceability and documentation available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a dry, moisture-free environment. This product is hygroscopic and light-sensitive; allow the sealed vial to equilibrate to room temperature before opening to prevent condensation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Moisture Content (KF)	≤ 1.0%
Solubility	Soluble in anhydrous acetonitrile

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.