Description

9-Fluorenylmethyl Chloroformate (CAS NO 28920-43-6) is a highly reactive and versatile reagent primarily used for the derivatization of amines and amino acids. Its core value lies in enabling sensitive and selective analysis by introducing the hydrophobic, UV-absorbing fluorenylmethyl (Fmoc) group, which is crucial for modern analytical and synthetic chemistry. This compound is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and analytical chemistry sectors who require high-purity reagents for peptide synthesis, amino acid analysis, and advanced proteomics workflows.

Application

• Fmoc-based Peptide Synthesis: Serves as a key reagent for introducing the 9-fluorenylmethoxycarbonyl (Fmoc) protecting group onto the N-terminus of amino acids, a fundamental step in solid-phase peptide synthesis (SPPS).
• Amino Acid Analysis (HPLC/LC-MS): Used to derivative primary and secondary amines, including amino acids, for enhanced detection via ultraviolet (UV) or fluorescence in liquid chromatography applications.
• Proteomics and Biomarker Research: Facilitates the labeling and quantification of peptides and proteins in complex biological samples for mass spectrometric analysis.
• Chiral Analysis and Separation: Employed to create diastereomeric derivatives of chiral amines for analysis by chiral chromatography or capillary electrophoresis.
• Pharmaceutical Intermediate: Acts as a building block in the synthesis of more complex molecules and active pharmaceutical ingredients (APIs) that require the Fmoc-protected amine functionality.
• Quality Control in Biologics: Applied in the characterization and purity assessment of therapeutic peptides and oligonucleotides.

Basic Information

Product Name	9-Fluorenylmethyl Chloroformate
CAS No.	28920-43-6
Molecular Formula	C15H11ClO2
Molecular Weight	258.70 g/mol
Synonyms	Fmoc-Cl; 9-Fluorenylmethyloxycarbonyl Chloride; N-(9-Fluorenylmethoxycarbonyloxy)succinimide; Chloroformic Acid 9-Fluorenylmethyl Ester; 9-Fluorenylmethyl Chlorocarbonate; 9-FMOC-Chloride; (9H-Fluoren-9-yl)methyl chloroformate; N-Fmoc Chloride
EINECS	249-309-3

Quality Control

Our 9-Fluorenylmethyl Chloroformate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity assessment and identity confirmation by IR and NMR spectroscopy, to ensure it meets the high standards required for sensitive research and production applications. Certificates of Analysis (COA) with detailed specifications are provided for every shipment to guarantee traceability and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry, and well-ventilated place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to prevent decomposition.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Melting Point	62 - 66 °C
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.