Description

(3β,22S)-Dihydroxy Cholesterol is a high-purity, multi-hydroxylated derivative of cholesterol, distinguished by its specific stereochemistry at the 3-beta and 22-S positions. This compound is a critical intermediate and reference standard in advanced biochemical research and pharmaceutical development, valued for its role in studying sterol metabolism and biosynthesis pathways. It is primarily utilized by research institutions, pharmaceutical R&D departments, and manufacturers of fine chemicals and diagnostic reagents.

Application

• Pharmaceutical Intermediate: Serves as a key chiral building block in the synthesis of complex steroid-based therapeutics and bioactive molecules.
• Biochemical Research: Used as a reference standard and substrate in enzymatic studies related to cholesterol metabolism, oxidation, and hydroxylation pathways.
• Analytical Standard: Essential for method development, calibration, and quality control in analytical techniques like HPLC and LC-MS for sterol analysis.
• Cell Biology Studies: Employed in research investigating membrane fluidity, lipid raft formation, and cellular signaling mechanisms involving modified sterols.
• Diagnostic Reagent Manufacturing: A potential component in the formulation of assay kits and calibrators for clinical diagnostics targeting lipid profiles.

Basic Information

Product Name	(3β,22S)-Dihydroxy Cholesterol
CAS No.	26649-05-8
Molecular Formula	C27H46O3
Molecular Weight	418.66 g/mol
Synonyms	22S-Hydroxycholesterol; (22S)-22-Hydroxycholesterol; 3β,22S-Dihydroxycholest-5-ene; Cholest-5-ene-3β,22S-diol; 22(S)-Hydroxycholesterol; 22S-HC; 22S-OHC; 5-Cholestene-3β,22S-diol
EINECS	Contact for details

Quality Control

Our (3β,22S)-Dihydroxy Cholesterol is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including identity confirmation by spectroscopic methods (IR, NMR) and purity assessment by HPLC to ensure it meets stringent specifications for research and industrial use. Certificates of Analysis (COA) with detailed batch-specific data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic (moisture-sensitive) nature, the container should be kept in a desiccated environment to maintain product integrity and prevent degradation.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 98.0%
Specific Rotation	Contact for details
Loss on Drying	≤ 1.0%
Residue on Ignition	≤ 0.1%
Single Unknown Impurity (HPLC)	≤ 0.5%
Total Impurities (HPLC)	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.