Description

20,21-Dihydroxycholesterol is a key oxysterol metabolite involved in the biosynthesis of bile acids and steroid hormones. This compound is of significant interest in biochemical research and pharmaceutical development due to its role as an intermediate in critical metabolic pathways. It is primarily utilized by researchers and manufacturers in the life sciences, pharmaceutical, and biotechnology sectors for analytical standards, metabolic studies, and as a precursor in synthesis.

Application

• Biochemical Research Standard: Used as a high-purity reference standard for analytical methods like HPLC and LC-MS in metabolic pathway studies.
• Pharmaceutical Intermediates: Serves as a critical intermediate in the synthesis of specialized steroid-based pharmaceuticals and active pharmaceutical ingredients (APIs).
• Metabolic Pathway Analysis: Employed in research to study cholesterol metabolism, bile acid biosynthesis, and related enzymatic processes.
• Cell Biology Studies: Utilized in in-vitro assays to investigate the biological activity and signaling roles of oxysterols.
• Diagnostic Reagent Development: Acts as a key component in the development of diagnostic kits and assays for metabolic disorders.

Basic Information

Product Name	20,21-Dihydroxycholesterol
CAS No.	26273-31-4
Molecular Formula	C27H46O3
Molecular Weight	418.66 g/mol
Synonyms	20,21-Dihydroxycholest-5-en-3β-ol; (3β)-Cholest-5-ene-3,20,21-triol; 20,21-Dihydroxycholest-5-en-3β-ol; 20,21-Dihydroxycholest-5-en-3-ol; 20,21-Dihydroxy-5-cholesten-3β-ol; 5-Cholestene-3β,20,21-triol; 20,21-Dihydroxycholesterol (major)
EINECS	Contact for details

Quality Control

Our 20,21-Dihydroxycholesterol is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the high standards required for research and development. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee traceability and batch-to-batch consistency.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a cool, dry place. The product is light-sensitive and should be handled accordingly to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 95.0%
Related Substances (HPLC)	Total impurities ≤ 5.0%
Loss on Drying	≤ 2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.