Description

5-Methyluridine Hemihydrate is a modified nucleoside derivative of uridine, featuring a methyl group at the 5-position of the pyrimidine ring. This specific molecular modification is crucial for research in nucleic acid chemistry, impacting base pairing and stability. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and life science sectors for applications in oligonucleotide synthesis, biochemical studies, and as a potential precursor for antiviral or anticancer prodrug development.

Application

• Oligonucleotide Synthesis: Serves as a key building block (phosphoramidite) for the solid-phase synthesis of modified RNA and DNA strands for research and therapeutic applications.
• Biochemical Research: Used as a substrate or inhibitor in enzymatic studies to investigate RNA metabolism, modification, and repair pathways.
• Pharmaceutical Intermediates: Acts as a critical precursor in the research and development of novel nucleoside-based antiviral or anticancer agents.
• Life Science Reagents: Incorporated into diagnostic kits and research reagents for studying gene expression and RNA biology.
• Reference Standard: Employed as a high-purity analytical standard in quality control (QC) and research laboratories for method development and validation.

Basic Information

Product Name	5-Methyluridine Hemihydrate
CAS No.	25954-21-6
Molecular Formula	C10H14N2O6 • ½H2O
Molecular Weight	261.23 g/mol (Anhydrous basis)
Synonyms	5-Methyluridine; 5-Methyl-Uridine; 1-((2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)oxolan-2-yl)-5-methylpyrimidine-2,4-dione Hemihydrate; rT; Ribothymidine; 5-Methyl-1-β-D-ribofuranosyluracil; 2,4(1H,3H)-Pyrimidinedione, 5-methyl-1-β-D-ribofuranosyl-
EINECS	Contact for details

Quality Control

Our 5-Methyluridine Hemihydrate is produced under a strict quality management system. Each batch undergoes comprehensive analytical testing including HPLC, NMR, and MS to ensure identity, purity, and consistency. Certificates of Analysis (COA) detailing all specifications are provided and available upon request, supporting compliance with research and cGMP-grade requirements where applicable.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to minimize exposure to atmospheric humidity.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥98.0%
Water Content (KF)	3.0% - 5.0% (Theoretical for hemihydrate: ~3.45%)
Related Substances (HPLC)	Total impurities ≤2.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.