Description

Adenylyl Imidodiphosphate is a non-hydrolyzable ATP analog, where a nitrogen atom replaces the oxygen atom in the bridging phosphate group. This compound is critical for studying ATP-dependent enzymes and processes, as it acts as a potent inhibitor by mimicking the transition state of ATP hydrolysis. It is an essential biochemical tool for researchers in enzymology, molecular biology, and structural biology, providing insights into kinase, ATPase, and synthase mechanisms without being degraded.

Application

• Enzyme Mechanism Studies: Used as a non-hydrolyzable substrate analog to trap and study the catalytic intermediates of ATP-dependent enzymes like kinases, ATPases, and synthases.
• Structural Biology: Employed in X-ray crystallography and cryo-EM to determine high-resolution structures of enzyme-ligand complexes.
• Biochemical Assay Development: Serves as a standard inhibitor in activity assays to characterize enzyme kinetics and inhibition constants (Ki).
• Signal Transduction Research: Utilized to investigate ATP-binding sites in G-proteins, motor proteins, and other signaling molecules.
• Pharmaceutical Screening: Acts as a tool compound in high-throughput screening for identifying potential therapeutic agents targeting ATP-binding pockets.
• Metabolic Pathway Analysis: Applied in studies of cellular energy metabolism and nucleotide-dependent regulatory pathways.

Basic Information

Product Name	Adenylyl Imidodiphosphate
CAS No.	25612-73-1
Molecular Formula	C10H17N6O12P3
Molecular Weight	506.19 g/mol
Synonyms	AMP-PNP; Adenylyl-imidodiphosphate; Adenosine 5'-(β,γ-imido)triphosphate; Adenosine 5'-[β,γ-imido]triphosphate; P1,P2-Di(adenosine-5')-5'-pentaphosphate; ATP analog (non-hydrolyzable); β,γ-Imido-ATP; Imidoadenosine triphosphate; 5'-Adenylyl imidodiphosphate
EINECS	Contact for details

Quality Control

Our Adenylyl Imidodiphosphate is produced under stringent quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry. We provide a detailed Certificate of Analysis (COA) with every shipment, ensuring traceability and compliance with research-grade specifications. Custom purity grades and bulk packaging are available to meet specific project requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. Due to its hygroscopic nature, the product should be handled under anhydrous conditions and allowed to equilibrate to room temperature before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, buffer solutions

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.