Description

5'-Deoxy-5'-[[Bis(2,2,2-Trichloroethoxy)Phosphinyl]Amino]Adenosine is a specialized nucleoside phosphoramidite derivative, a critical building block in oligonucleotide synthesis. Its primary value lies in enabling the precise and automated construction of DNA and RNA sequences, particularly for introducing modified bases. This high-purity intermediate is essential for research institutions, diagnostic developers, and pharmaceutical companies engaged in antisense therapy, siRNA development, and molecular biology applications.

Application

• Oligonucleotide Synthesis: A key phosphoramidite monomer for the solid-phase synthesis of custom DNA and RNA strands.
• Antisense Drug Development: Used in the research and production of antisense oligonucleotides (ASOs) for therapeutic applications.
• siRNA and miRNA Synthesis: Critical for constructing short interfering RNA (siRNA) and microRNA (miRNA) molecules for gene silencing studies.
• Molecular Probes and Primers: Manufacture of labeled probes, primers, and other tools for genetic analysis and diagnostics.
• Structural Biology Studies: Incorporation into oligonucleotides to study nucleic acid structure, function, and protein interactions.
• High-Throughput Screening Libraries: Production of diverse oligonucleotide libraries for drug discovery and genomic research.

Basic Information

Product Name	5'-Deoxy-5'-[[Bis(2,2,2-Trichloroethoxy)Phosphinyl]Amino]Adenosine
CAS No.	25254-10-8
Molecular Formula	C₁₆H₁₉Cl₆N₆O₆P
Molecular Weight	642.99 g/mol
Synonyms	5'-Amino-5'-deoxyadenosine N-(bis(2,2,2-trichloroethoxy)phosphinyl) derivative; Adenosine, 5'-deoxy-5'-((bis(2,2,2-trichloroethoxy)phosphinyl)amino)-; 5'-Deoxy-5'-(N-(bis(2,2,2-trichloroethoxy)phosphinyl)amino)adenosine; TCE-Protected Adenosine Phosphoramidite; 5'-Amino Modifier Phosphoramidite (Adenosine); N-TCE Phosphoramidite of 5'-Amino-5'-deoxyadenosine
EINECS	Contact for details

Quality Control

This high-value synthetic intermediate is produced under strict quality control protocols to ensure batch-to-batch consistency and reliability for sensitive synthesis workflows. Each lot is characterized by HPLC, NMR, and mass spectrometry to confirm identity and purity. A comprehensive Certificate of Analysis (COA) detailing purity, moisture content, and solvent residues is provided with every shipment. Our manufacturing process adheres to cGMP principles where applicable, supporting its use in research and development leading to therapeutic applications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to equilibrate to room temperature before opening to prevent moisture condensation. For long-term stability, store in the original packaging.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%
Solvent Residues (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.