Description

5'-Deoxy-5'-[Bis(2,2,2-Trichloroethoxy)Phosphinylamino]Thymidine is a specialized nucleoside phosphoramidite derivative, a critical building block in the synthesis of oligonucleotides. Its primary value lies in enabling the precise, automated construction of DNA and RNA sequences for research and therapeutic development. This high-purity intermediate is essential for pharmaceutical R&D, biotechnology companies, and academic laboratories focused on antisense technology, siRNA, and diagnostic probe development.

Application

• Solid-Phase Oligonucleotide Synthesis: As a key phosphoramidite monomer for the automated, stepwise assembly of custom DNA and RNA strands.
• Antisense Oligonucleotide (ASO) Production: For manufacturing therapeutic oligonucleotides designed to target specific RNA sequences to modulate gene expression.
• siRNA and miRNA Synthesis: Used in the creation of short interfering RNA (siRNA) and microRNA (miRNA) molecules for gene silencing research and RNAi-based therapeutics.
• Diagnostic Probe Development: Enables the production of labeled DNA probes for applications in PCR, microarrays, and fluorescence in situ hybridization (FISH).
• Research-Grade Oligo Synthesis: Supplies the needs of academic and industrial R&D labs for high-quality, sequence-specific oligonucleotides.
• Pharmaceutical Intermediate: Serves as a cGMP-ready starting material for the commercial-scale production of oligonucleotide active pharmaceutical ingredients (APIs).

Basic Information

Product Name	5'-Deoxy-5'-[Bis(2,2,2-Trichloroethoxy)Phosphinylamino]Thymidine
CAS No.	25152-21-0
Molecular Formula	C14H18Cl6N3O7P
Molecular Weight	584.91 g/mol
Synonyms	5'-Deoxy-5'-(N,N-bis(2,2,2-trichloroethoxy)phosphinylamino)thymidine; Thymidine, 5'-deoxy-5'-[bis(2,2,2-trichloroethoxy)phosphinylamino]-; 5'-Amino-5'-deoxythymidine N,N-bis(2,2,2-trichloroethyl) phosphoramidite; dT Phosphoramidite (2,2,2-Trichloroethyl protected); CE-T Phosphoramidite; Thymidine Phosphoramidite (TCE protected); 5'-Deoxythymidine Phosphoramidite Derivative
EINECS	Contact for details

Quality Control

Our 5'-Deoxy-5'-[Bis(2,2,2-Trichloroethoxy)Phosphinylamino]Thymidine is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for oligonucleotide synthesis. We provide comprehensive Certificates of Analysis (COA) with each batch, detailing purity, identity, and moisture content. Production can adhere to cGMP guidelines for pharmaceutical intermediate applications upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as specified on the label. This material is hygroscopic (moisture-sensitive) and should be handled under an inert atmosphere (argon or nitrogen) when opened to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference
Assay (HPLC)	≥ 97.0%
Water Content (KF)	≤ 0.5%
Solubility	Soluble in anhydrous acetonitrile, DCM

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.