Description

Alpha,β-Methylenedeoxythymidine 5'-Triphosphate is a chemically modified nucleotide triphosphate analog, specifically engineered to act as a chain terminator in nucleic acid synthesis. This compound is critical for applications requiring precise control over DNA sequencing and polymerase activity. It is primarily utilized by researchers and manufacturers in the fields of molecular biology, diagnostics, and pharmaceutical development.

Application

• DNA Sequencing: Acts as a specific chain-terminating substrate in Sanger sequencing and next-generation sequencing (NGS) platforms.
• Enzyme Studies: Used as a tool to investigate the kinetics and mechanisms of DNA polymerases and reverse transcriptases.
• Antiviral Research: Serves as a potential nucleotide analog for studying the inhibition of viral replication.
• Diagnostic Assay Development: Incorporated into probes and primers for highly specific detection systems.
• Synthetic Biology: Employed in the engineering of novel enzymatic pathways and genetic circuits.
• Biochemical Reagent Manufacturing: A key raw material for producing high-precision kits for molecular biology applications.

Basic Information

Product Name	Alpha,β-Methylenedeoxythymidine 5'-Triphosphate
CAS No.	24716-71-0
Molecular Formula	C11H17N2O13P3
Molecular Weight	502.17 g/mol
Synonyms	2',3'-Didehydro-2',3'-dideoxythymidine 5'-triphosphate; D4T-TP; Stavudine triphosphate; 1-((2R,4S,5S)-4-Hydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methyl-2,4(1H,3H)-pyrimidinedione 5'-(trihydrogen triphosphate); (E)-2',3'-Didehydro-3'-deoxythymidine 5'-triphosphate
EINECS	Contact for details

Quality Control

Our Alpha,β-Methylenedeoxythymidine 5'-Triphosphate is produced under stringent quality management systems. Each batch is subjected to comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods. Certificates of Analysis (COA) detailing purity, impurity profile, and other critical parameters are provided to ensure compliance with your research and manufacturing standards.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/UV)	Conforms to reference standard
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water or suitable aqueous buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.