Description

Thymidine-3’,5’-Di(p-Nitrophenyl Phosphate) Disodium Salt is a high-purity biochemical reagent and a key nucleotide derivative. This compound is primarily valued as a critical substrate and intermediate in enzymatic research and nucleic acid chemistry. It is essential for researchers and manufacturers in the fields of molecular biology, pharmaceutical development, and diagnostic assay production, where precise biochemical tools are required.

Application

• Enzymatic Substrate: Used as a specific substrate for studying the activity of nucleases, phosphatases, and other DNA-modifying enzymes.
• Nucleic Acid Synthesis: Serves as a key phosphorylated intermediate in the chemical and enzymatic synthesis of oligonucleotides and their analogs.
• Biochemical Research: Employed in mechanistic studies of DNA repair, replication, and phosphorylation pathways.
• Diagnostic Reagent Manufacturing: Utilized in the production of kits and reagents for molecular diagnostics and life science research.
• Pharmaceutical Intermediate: Acts as a building block in the development of novel antiviral and anticancer nucleotide-based therapeutics.
• Enzyme Kinetics & Inhibition Studies: Provides a chromogenic or fluorogenic readout (via p-nitrophenol release) for high-throughput screening and kinetic analysis.

Basic Information

Product Name	Thymidine-3’,5’-Di(p-Nitrophenyl Phosphate) Disodium Salt
CAS No.	24418-12-0
Molecular Formula	C22H20N4Na2O14P2
Molecular Weight	672.33 g/mol
Synonyms	Thymidine 3',5'-Bis(p-nitrophenyl phosphate) Disodium Salt; p-Nitrophenyl Thymidine 3',5'-Diphosphate Disodium Salt; Thymidine 3',5'-Di-p-nitrophenyl Phosphate Disodium Salt; 3',5'-Di-O-(p-nitrophenyl)phosphorylthymidine Disodium Salt; TpNppNa2; Thymidine-3',5'-cyclic monophosphate p-nitrophenyl ester disodium salt; Thymidine 3',5'-cyclic monophosphate p-nitrophenyl ester sodium salt
EINECS	Contact for details

Quality Control

Our Thymidine-3’,5’-Di(p-Nitrophenyl Phosphate) Disodium Salt is produced under stringent quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry, to ensure it meets the exacting standards required for life science research. A detailed Certificate of Analysis (COA) is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent degradation from atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in water, DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.