Description

5'-O-(4,4'-Dimethoxytrityl)-2'-Deoxyuridine is a key protected nucleoside derivative, essential for the controlled synthesis of oligonucleotides. This compound is critical for ensuring high coupling efficiency and sequence fidelity in solid-phase DNA synthesis, particularly for the introduction of thymidine analogs. It is primarily utilized by manufacturers and research institutions in the pharmaceutical, biotechnology, and diagnostic sectors for the development of antisense oligonucleotides, primers, probes, and other nucleic acid-based therapeutics.

Application

• Solid-Phase Oligonucleotide Synthesis (SPOS): Serves as a crucial building block (phosphoramidite precursor) for the automated synthesis of DNA strands.
• Antisense Oligonucleotide (ASO) Production: Used in the manufacture of therapeutic ASOs targeting specific mRNA sequences for treating genetic disorders and cancers.
• Diagnostic Probe & Primer Synthesis: Enables the production of labeled or modified probes and primers for PCR, sequencing, and microarray applications.
• Research & Development: A fundamental reagent in academic and industrial R&D for studying nucleic acid chemistry, enzyme mechanisms, and developing novel bioconjugates.
• Synthesis of Modified Nucleosides: Acts as a protected intermediate for further chemical modifications to create nucleoside analogs with enhanced properties.

Basic Information

Product Name	5'-O-(4,4'-Dimethoxytrityl)-2'-Deoxyuridine
CAS No.	23669-79-6
Molecular Formula	C30H30N2O7
Molecular Weight	530.57 g/mol
Synonyms	5'-O-DMT-2'-deoxyuridine; DMT-dU; 2'-Deoxy-5'-O-(4,4'-dimethoxytrityl)uridine; 1-[(2R,4S,5R)-4-Hydroxy-5-(hydroxymethyl)oxolan-2-yl]-5-(4,4-dimethoxytrityloxymethyl)pyrimidine-2,4-dione; NSC 332910; 5'-O-(Bis(4-methoxyphenyl)phenylmethoxy)-2'-deoxyuridine
EINECS	Contact for details

Quality Control

Our 5'-O-(4,4'-Dimethoxytrityl)-2'-Deoxyuridine is manufactured under strict quality systems to ensure it meets the exacting standards required for nucleic acid synthesis. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to cGMP principles where applicable, and our quality protocols are designed to support regulatory filings for pharmaceutical applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions or a dry inert atmosphere to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms to reference standard
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Related Substances (HPLC)	Total impurities ≤ 2.0% Any single impurity ≤ 1.0%
Solubility	Soluble in common organic solvents (e.g., DCM, DMF, Acetonitrile)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.