Description

8-Bromoguanosine Triphosphate (CAS NO 23197-98-0) is a chemically modified nucleotide triphosphate analog essential for advanced molecular biology and biochemical research. Its core value lies in its ability to act as a substrate for DNA and RNA polymerases, enabling the site-specific incorporation of bromine-labeled guanosine into nucleic acid chains. This product is critically needed by researchers in pharmaceutical development, molecular diagnostics, and structural biology for applications such as X-ray crystallography, cross-linking studies, and the synthesis of modified oligonucleotides.

Application

• Nucleic Acid Labeling & Cross-linking: Used as a substrate for polymerases to incorporate bromine atoms into DNA or RNA for photo-cross-linking experiments and structural analysis.
• X-ray Crystallography: Serves as a heavy atom derivative (bromine) to solve the phase problem in determining the three-dimensional structures of nucleic acids and protein-nucleic acid complexes.
• Enzyme Mechanism Studies: Employed to investigate the substrate specificity and catalytic mechanisms of DNA/RNA polymerases, kinases, and other nucleotide-processing enzymes.
• Modified Oligonucleotide Synthesis: A key building block for the enzymatic synthesis of bromine-labeled oligonucleotides used as probes or for studying nucleic acid interactions.
• SELEX (Systematic Evolution of Ligands by Exponential Enrichment): Can be used to generate modified aptamer libraries with enhanced stability or binding properties.
• Biophysical Probes: Utilized in fluorescence quenching, FRET (Förster Resonance Energy Transfer), and other biophysical assays due to the heavy atom effect of bromine.

Basic Information

Product Name	8-Bromoguanosine Triphosphate
CAS No.	23197-98-0
Molecular Formula	C10H15BrN5O14P3
Molecular Weight	602.10 g/mol
Synonyms	8-Br-GTP; 8-Bromo-GTP; Guanosine 5'-(tetrahydrogen triphosphate), 8-bromo-; 8-Bromoguanosine 5'-triphosphate; Br8GTP; 8-Bromo-5'-GTP; 8-Bromoguanosine-5'-triphosphate
EINECS	Contact for details

Quality Control

Our 8-Bromoguanosine Triphosphate is manufactured under stringent conditions to ensure high purity and batch-to-batch consistency suitable for sensitive research applications. Each lot undergoes rigorous analytical testing, including HPLC for purity assessment and identity confirmation. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below. This compound is hygroscopic (moisture-sensitive). For long-term storage, desiccate at -20°C. Allow the product to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC/UV)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5%
Solubility	Soluble in water, buffer solutions
Biological Activity	Suitable for polymerase incorporation

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.