Description

22(R)-Hydroxycholesterol CAS NO 22348-64-7 is a high-purity, stereospecific oxysterol derivative of cholesterol. This compound serves as a critical biochemical tool and a key intermediate in the research of lipid metabolism and signaling pathways. It is essential for researchers and manufacturers in the pharmaceutical, biotechnology, and life science sectors, particularly those focused on studying liver X receptor (LXR) activity, cholesterol homeostasis, and neurosteroid synthesis.

Application

• Biochemical Research: Used as a reference standard and analytical reagent in studies of cholesterol biosynthesis and metabolism.
• Pharmaceutical R&D: A key intermediate in the synthesis of more complex steroid-based drug candidates targeting nuclear receptors.
• Cell Signaling Studies: Employed to investigate the role of oxysterols as ligands for Liver X Receptors (LXRs) and their impact on gene expression related to lipid and glucose metabolism.
• Neuroscience Research: Utilized in studies exploring the role of hydroxycholesterols in the brain, including neuroprotection and neurosteroid production.
• Diagnostic Development: Serves as a calibration standard in chromatographic (HPLC, GC-MS) and spectroscopic methods for quantifying sterol profiles in biological samples.
• Enzymology: Acts as a substrate for studying the activity and kinetics of specific cytochrome P450 enzymes and other hydroxylases.

Basic Information

Product Name	22(R)-Hydroxycholesterol
CAS No.	22348-64-7
Molecular Formula	C27H46O2
Molecular Weight	402.66 g/mol
Synonyms	(22R)-Cholest-5-ene-3β,22-diol; 22R-Hydroxycholesterol; (3β,22R)-Cholest-5-ene-3,22-diol; 22(R)-OH-Cholesterol; 22R-OHC; 22R-HC; 5-Cholestene-3β,22(R)-diol; 22(R)-Hydroxy-cholesterol
EINECS	Contact for details

Quality Control

Our 22(R)-Hydroxycholesterol is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets the stringent requirements of research and development applications. A Certificate of Analysis (COA) detailing purity, specific rotation, and chromatographic data is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere for long-term stability. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to warm to room temperature before opening to minimize moisture condensation. For short-term use, store desiccated at 2-8°C.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Specific Rotation	Contact for details
Loss on Drying	≤ 1.0%
Single Impurity (HPLC)	≤ 0.5%
Solubility	Soluble in ethanol, DMSO; slightly soluble in chloroform

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.