Description

3β,12β,14-Trihydroxybufa-4,20,22-Trienolide is a specialized bufadienolide compound, a class of cardioactive steroids with significant biological activity. This high-purity reference standard is critical for pharmaceutical research and development, particularly in the study of cardiac glycosides and their therapeutic mechanisms. It is primarily utilized by research institutions, pharmaceutical companies, and analytical laboratories for method development, quality control, and biological activity studies.

Application

• Pharmaceutical Reference Standard: Serves as a certified primary standard for the identification, assay, and purity testing of bufadienolide-based drug substances and products.
• Biochemical Research: Used in pharmacological studies to investigate the effects on sodium-potassium ATPase activity, cardiac contractility, and potential anti-cancer properties.
• Analytical Chemistry: Employed as a high-purity calibrant in chromatographic methods (HPLC, LC-MS) for the quantitative analysis of complex natural product extracts.
• Natural Product Isolation & Characterization: Acts as a key marker compound for the quality assessment and standardization of toad venom-derived traditional medicines and related botanical preparations.
• Toxicology Studies: Utilized in the development and validation of analytical assays for the detection and quantification of cardioactive steroids in forensic and clinical toxicology.

Basic Information

Product Name	3β,12β,14-Trihydroxybufa-4,20,22-Trienolide
CAS No.	21305-04-4
Molecular Formula	C₂₄H₃₂O₆
Molecular Weight	416.51 g/mol
Synonyms	3β,12β,14-Trihydroxybufa-4,20,22-trienolide; Bufalin-3,12,14-triol; 14β,15β-Epoxy-3β,12β,14β-bufa-4,20,22-trienolide; Telocinobufagin 3-acetate (related); Bufadienolide C-1; Resibufogenin trihydroxy derivative; (3β,12β,14β)-3,12,14-Trihydroxybufa-4,20,22-trienolide
EINECS	Contact for details

Quality Control

Our 3β,12β,14-Trihydroxybufa-4,20,22-Trienolide is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, IR and NMR for structural confirmation, and residual solvent analysis. A detailed Certificate of Analysis (COA) is provided, documenting identity, purity, and all relevant specifications to ensure compliance with research and pharmaceutical-grade standards.

Storage

Preserve in a tightly closed container, protected from light. Store at controlled room temperature (15-25°C) in a dry environment. The product is light-sensitive and should be handled under appropriate conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time corresponds to reference standard
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 0.5%
Residue on Ignition	≤ 0.1%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.