Description

3'-(o-Methyl)Cytidine is a modified nucleoside derivative where a methyl group is introduced at the ortho position of the cytidine base. This specific structural modification is crucial for fine-tuning the properties of oligonucleotides, impacting their stability, binding affinity, and resistance to enzymatic degradation. It is primarily utilized by researchers and manufacturers in the pharmaceutical and biotechnology sectors for advanced nucleic acid synthesis, including the development of therapeutic oligonucleotides, antisense agents, and diagnostic probes.

Application

• Therapeutic Oligonucleotide Synthesis: A key building block for manufacturing modified antisense oligonucleotides (ASOs) and siRNA therapeutics to enhance metabolic stability and target engagement.
• Research & Diagnostic Probe Development: Used in the creation of labeled probes and primers for advanced molecular biology research, diagnostics, and genomic studies.
• Medicinal Chemistry & Drug Discovery: Serves as a critical intermediate in medicinal chemistry programs aimed at developing novel nucleoside-based antiviral or anticancer agents.
• Bioconjugation and Labeling: Facilitates the site-specific modification and conjugation of oligonucleotides with reporter molecules or delivery vehicles.
• Structural Studies of Nucleic Acids: Employed to study the structure-function relationship of RNA and DNA by introducing specific steric or electronic modifications.

Basic Information

Product Name	3'-(o-Methyl)Cytidine
CAS No.	20594-00-7
Molecular Formula	C10H15N3O5
Molecular Weight	257.24 g/mol
Synonyms	3'-O-Methylcytidine; 1-(3-O-Methyl-β-D-ribofuranosyl)cytosine; Cytidine, 3'-O-methyl-; o-Methylcytidine; 3'-Methylcytidine; N4-Amino-1-(3-O-methyl-β-D-ribofuranosyl)-2(1H)-pyrimidinone; 3'-OMe-Cytidine
EINECS	Contact for details

Quality Control

Our 3'-(o-Methyl)Cytidine is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and tests for residual solvents and moisture. We provide a detailed Certificate of Analysis (COA) with every shipment to ensure traceability and compliance with your research or cGMP-grade requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as specified on the label or COA. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.