Description

27-Hydroxycholesterol is a significant endogenous oxysterol and a key metabolite in the cholesterol biosynthetic pathway. This compound is of critical importance in biomedical research as a selective endogenous estrogen receptor modulator and a biomarker for various disease states. It is primarily utilized by researchers and manufacturers in the pharmaceutical, biotechnology, and diagnostic sectors for studying atherosclerosis, cancer biology, and neurodegenerative disorders.

Application

• Biomedical Research: Used as a reference standard and active agent in studies of cholesterol metabolism, liver X receptor (LXR) signaling, and estrogen receptor activity.
• Pharmaceutical Development: Serves as a critical intermediate or biomarker in the development of therapeutics targeting cardiovascular diseases, breast cancer, and Alzheimer's disease.
• Diagnostic Assays: Employed as a calibration standard in LC-MS/MS and ELISA kits for the quantitative analysis of oxysterols in biological samples (plasma, serum, tissues).
• Cell Biology Studies: Applied in *in vitro* cell culture models to investigate its effects on cell proliferation, apoptosis, and inflammatory responses.
• Biochemical Reagent: Used as a substrate or inhibitor in enzymatic studies related to cytochrome P450 enzymes (CYP27A1) and hydroxysteroid dehydrogenases.

Basic Information

Product Name	27-Hydroxycholesterol
CAS No.	20380-11-4
Molecular Formula	C27H46O2
Molecular Weight	402.66 g/mol
Synonyms	(25R)-26-Hydroxycholesterol; 5-Cholestene-3β,26-diol; 27-HC; 26-Hydroxycholesterol; Cholest-5-ene-3β,26-diol; (3β,25R)-Cholest-5-ene-3,26-diol; 27-OHC
EINECS	243-799-3

Quality Control

Our 27-Hydroxycholesterol is produced under strict quality management systems. Each batch undergoes comprehensive analytical testing including HPLC for purity, identity confirmation by IR and NMR spectroscopy, and specific tests for related substances. Certificates of Analysis (COA) detailing all specifications and test results are provided to ensure traceability and compliance with your research or production requirements.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C under an inert atmosphere (e.g., argon or nitrogen) to prevent oxidation. Allow the product to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 0.5%
Specific Rotation	Contact for details
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.