Description

5-Bromouridine-5'-Monophosphate is a modified nucleotide monophosphate where a bromine atom is substituted at the 5-position of the uracil base. This modification is critical for its role as a versatile building block in nucleic acid research and as a key intermediate in the synthesis of more complex nucleoside analogs. It is primarily utilized by research institutions and pharmaceutical companies engaged in oligonucleotide synthesis, biochemical studies, and antiviral drug development. The compound's unique structure allows it to act as a probe or inhibitor in enzymatic studies related to RNA metabolism.

Application

• Oligonucleotide Synthesis: Used as a modified phosphoramidite or solid-phase synthesis building block to incorporate brominated bases into DNA or RNA strands for structural studies, cross-linking experiments, or to modulate duplex stability.
• Biochemical Research: Serves as a substrate or inhibitor for enzymes such as kinases, phosphorylases, and polymerases to study nucleotide metabolism and enzyme mechanism.
• Antiviral Drug Development: Acts as a crucial intermediate in the synthetic pathway of investigational nucleoside analog prodrugs targeting viral polymerases.
• RNA Labeling and Detection: Can be used to introduce a heavy atom or a reactive handle into RNA molecules for detection via specialized techniques or structural analysis.
• Reference Standard: Employed as a high-purity analytical standard in quality control laboratories for HPLC or LC-MS method development and validation.

Basic Information

Product Name	5-Bromouridine-5'-Monophosphate
CAS No.	2149-79-3
Molecular Formula	C9H12BrN2O9P
Molecular Weight	403.08 g/mol
Synonyms	5-BrUMP; 5-Bromouridylic acid; 5-Bromo-UMP; 5-Bromouridine 5'-(dihydrogen phosphate); Uridine, 5-bromo-, 5'-(dihydrogen phosphate); 5-Bromouridine monophosphate; BrdUMP; 5-Bromo-2'-deoxyuridine-5'-monophosphate (common misnomer, verify structure)
EINECS	218-406-1

Quality Control

Our 5-Bromouridine-5'-Monophosphate is manufactured under a quality management system and undergoes rigorous analytical testing to ensure identity, purity, and consistency. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing specifications such as assay (HPLC), related substances, and residual solvents. We support research and development with material suitable for use as a reference standard, and specifications can be aligned with customer-specific requirements upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to prevent decomposition. Allow the container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Related Substances (HPLC)	Total impurities ≤ 2.0%
Water Content (KF)	≤ 5.0%
Heavy Metals	≤ 20 ppm
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.