Description

N2-Methylguanosine CAS NO 2140-77-4 is a modified nucleoside where a methyl group is attached to the N2 position of the guanine base. This specific methylation pattern is of significant interest in biochemical research, particularly in the study of RNA modification and epigenetic regulation. It serves as a critical reference standard and building block for researchers in pharmaceutical development, molecular biology, and analytical chemistry. The compound's defined structure is essential for investigating cellular processes and developing novel therapeutic agents.

Application

• Biochemical Research Standard: Used as a high-purity reference material for the identification and quantification of RNA modifications in mass spectrometry and HPLC analysis.
• Epigenetics & RNA Biology: A vital tool for studying the role and function of N2-methylguanosine in transfer RNA (tRNA) and messenger RNA (mRNA), impacting translation efficiency and gene expression.
• Pharmaceutical R&D: Employed as a key intermediate or analytical standard in the development of nucleoside-based therapeutics, including antiviral and anticancer agents.
• Enzymology Studies: Serves as a substrate or inhibitor for methyltransferase enzymes to understand catalytic mechanisms and for drug discovery screening.
• Diagnostic Kit Development: Utilized in the formulation of calibration standards for diagnostic assays targeting modified nucleosides, which can be biomarkers for certain diseases.
• Synthetic Biology: Acts as a building block for the synthesis of modified oligonucleotides to create novel RNA molecules with tailored properties.

Basic Information

Product Name	N2-Methylguanosine
CAS No.	2140-77-4
Molecular Formula	C11H15N5O5
Molecular Weight	297.27 g/mol
Synonyms	2'-Deoxy-2'-methylguanosine; N(2)-Methylguanosine; 1-[(2R,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-2-(methylamino)-1H-purin-6-one; 2-Methylaminoguanosine; m2G; N2-Methylguanosine riboside
EINECS	218-376-7

Quality Control

Our N2-Methylguanosine is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via NMR and mass spectrometry, to ensure it meets the high standards required for research and development. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to guarantee traceability and consistency for your critical applications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Residue on Ignition	≤ 0.5%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.