Description

2'-O-Methylcytidine is a chemically modified nucleoside where a methyl group is attached to the 2' oxygen of the ribose sugar. This modification is critical for enhancing the stability and biological activity of oligonucleotides, making it a valuable building block in advanced therapeutic and diagnostic platforms. It is primarily utilized by researchers and manufacturers in the pharmaceutical and biotechnology sectors for the development of next-generation nucleic acid-based drugs, including antisense oligonucleotides and siRNA.

Application

• Pharmaceutical Intermediate: A key raw material in the synthesis of modified oligonucleotides for therapeutic applications.
• Antisense Oligonucleotide (ASO) Development: Used to create nuclease-resistant RNA strands with improved pharmacokinetic profiles.
• siRNA and miRNA Synthesis: Incorporated to enhance stability and specificity of small interfering RNA and microRNA constructs.
• Diagnostic Probe Manufacturing: Serves as a component in the production of stable, high-affinity nucleic acid probes for molecular diagnostics.
• Biochemical Research: Employed in studies investigating RNA structure, function, and protein-RNA interactions.
• Vaccine Development: Investigated for use in mRNA vaccine platforms to modulate immunogenicity and stability.

Basic Information

Product Name	2'-O-Methylcytidine
CAS No.	2140-72-9
Molecular Formula	C10H15N3O5
Molecular Weight	257.24 g/mol
Synonyms	2'-O-Methylcytidine; 1-[(2R,3R,4R,5R)-3,4-Dihydroxy-5-(hydroxymethyl)-3-methyloxolan-2-yl]-4-aminopyrimidin-2-one; Cytidine, 2'-methyl-; 2'-Methylcytidine; 4-Amino-1-((2R,3R,4R,5R)-3,4-dihydroxy-5-(hydroxymethyl)-3-methyltetrahydrofuran-2-yl)pyrimidin-2(1H)-one; 2'-OMe-C; N4-Amino-1-(2-O-methyl-β-D-ribofuranosyl)cytosine
EINECS	218-377-1

Quality Control

Our 2'-O-Methylcytidine is produced under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation via IR and NMR, to ensure it meets the high standards required for pharmaceutical research and production. Certificates of Analysis (COA) detailing all specifications are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive) and must be kept under dry conditions to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference standard
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥98.0%
Water Content (KF)	≤1.0%
Residue on Ignition	≤0.5%
Heavy Metals	≤20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.