Description

2'-O-Methylpseudouridine is a chemically modified nucleoside analog that serves as a critical building block for advanced nucleic acid therapeutics. This compound is essential for enhancing the stability, translational efficiency, and immunogenicity profile of synthetic mRNA constructs. It is primarily utilized by pharmaceutical developers, biotechnology companies, and research institutions engaged in the innovation of mRNA-based vaccines, protein replacement therapies, and other cutting-edge genetic medicines.

Application

• mRNA Vaccine & Therapeutic Development: A key component for synthesizing modified mRNA to improve efficacy and reduce innate immune response.
• Nucleic Acid Research: Used in biochemical studies to investigate RNA structure, function, and stability.
• Antisense Oligonucleotide (ASO) Synthesis: Incorporated into oligonucleotides to modulate RNA-targeting activity and pharmacokinetics.
• CRISPR/Cas9 System Delivery: Enhances the performance of mRNA components in gene editing tools.
• Cell Reprogramming & Differentiation: Employed in mRNA cocktails for induced pluripotent stem cell (iPSC) generation and directed differentiation.
• Therapeutic Protein Production: Integral to mRNA sequences designed for in vivo production of therapeutic proteins.

Basic Information

Product Name	2'-O-Methylpseudouridine
CAS No.	2140-68-3
Molecular Formula	C10H14N2O6
Molecular Weight	258.23 g/mol
Synonyms	2'-O-Methyl-5-ribosyluracil; 5-(2-O-Methyl-β-D-ribofuranosyl)uracil; Pseudouridine, 2'-O-methyl-; 2'-OMe-ψ; m2'ψ; 2'-O-Methyl-ψ; ψm; 2'-O-Methyl-5-β-D-ribofuranosyluracil
EINECS	Contact for details

Quality Control

Our 2'-O-Methylpseudouridine is manufactured under strict quality management systems to ensure it meets the exacting standards required for pharmaceutical and biotechnological applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We support compliance with cGMP, ICH Q7 guidelines, and relevant pharmacopeial standards to ensure supply chain integrity for critical research and development.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as specified on the label. This material is hygroscopic (moisture-sensitive) and should be handled under dry conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC/IR)	Conforms
Assay (HPLC)	≥98.0%
Purity (HPLC, Area%)	≥99.0%
Water Content (KF)	≤1.0%
Residue on Ignition	≤0.1%
Heavy Metals	≤10 ppm
Bacterial Endotoxins	<1.0 EU/mg
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.