Description

3-Methylcytidine is a modified nucleoside where a methyl group is attached to the cytosine base. This structural modification is of significant interest in biochemical research, particularly in the study of RNA modification, epigenetics, and nucleic acid metabolism. It serves as a critical building block and reference standard for pharmaceutical R&D, diagnostic assay development, and advanced life science studies. Key industries that utilize this compound include biotechnology, academic research, and pharmaceutical development.

Application

• Biochemical Research: Used as a reference standard and reagent in studies of RNA methylation, tRNA modification, and epigenetic regulation.
• Pharmaceutical Development: Serves as a key intermediate or analytical standard in the synthesis and quality control of nucleoside-based therapeutics.
• Diagnostic Assays: Employed in the development and calibration of diagnostic kits for detecting nucleic acid modifications.
• Enzymology Studies: Acts as a substrate or inhibitor for enzymes involved in nucleotide metabolism and modification, such as methyltransferases.
• Life Science Tools: Incorporated into oligonucleotide synthesis for creating probes with specific properties for molecular biology applications.
• Metabolite Analysis: Used as a standard in mass spectrometry and chromatography for the identification and quantification of modified nucleosides in biological samples.

Basic Information

Product Name	3-Methylcytidine
CAS No.	2140-64-9
Molecular Formula	C10H15N3O5
Molecular Weight	257.24 g/mol
Synonyms	3-Methylcytidine; 3-Methylcytosine riboside; 1-β-D-Ribofuranosyl-3-methylcytosine; 3-MCyd; m³C; N3-Methylcytidine; Cytidine, 3-methyl-; 3-Methyl-1-β-D-ribofuranosylcytosine
EINECS	Contact for details

Quality Control

Our 3-Methylcytidine is produced under a strict quality management system. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and tests for residual solvents and moisture. We provide Certificates of Analysis (COA) with detailed specifications for traceability and quality assurance, supporting research and development requiring high-purity biochemicals.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; therefore, containers must be kept tightly sealed and under appropriate conditions to maintain stability and purity.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Heavy Metals	≤ 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.