Description

5-Methylcytidine is a modified nucleoside where a methyl group is attached to the cytosine base. This modification is crucial for research into epigenetic regulation, RNA biology, and nucleic acid metabolism. It serves as a vital biochemical tool and building block for scientists in pharmaceutical development, life science research, and diagnostic applications. Our high-purity grade ensures reliable and reproducible results for your most demanding projects.

Application

• Epigenetics and Molecular Biology Research: Used as a standard and reagent in studies of DNA and RNA methylation, a key epigenetic marker.
• Nucleic Acid Synthesis: Acts as a protected intermediate or monomer in the chemical and enzymatic synthesis of modified oligonucleotides.
• Pharmaceutical R&D: Investigated for potential applications in antiviral and anticancer drug development targeting nucleic acid pathways.
• Biochemical Assays: Employed as a substrate or inhibitor in enzymatic studies involving nucleoside kinases, methyltransferases, and other nucleic acid-modifying enzymes.
• Diagnostic Development: Utilized in the creation of probes and assays for detecting specific methylation patterns linked to diseases.
• Metabolic Pathway Studies: A key compound for tracing and understanding the salvage pathways of nucleoside metabolism.

Basic Information

Product Name	5-Methylcytidine
CAS No.	2140-61-6
Molecular Formula	C10H15N3O5
Molecular Weight	257.24 g/mol
Synonyms	5-Methylcytidine; 5-MCyd; 4-Amino-1-((2R,3R,4S,5R)-3,4-dihydroxy-5-(hydroxymethyl)tetrahydrofuran-2-yl)-5-methylpyrimidin-2(1H)-one; 1-β-D-Ribofuranosyl-5-methylcytosine; 5-Methylcytosine riboside; m5C; NSC 406024
EINECS	218-377-5

Quality Control

Our 5-Methylcytidine is manufactured under a strict quality management system. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and tests for residual solvents and moisture. A Certificate of Analysis (COA) detailing all specifications is provided to ensure traceability and compliance with your research or production standards.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C for long-term stability. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Heavy Metals	≤ 10 ppm
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.