Description

5'-Dmt-N4-Ac-5-Me-Dc Phosphoramidite is a high-purity, protected nucleoside phosphoramidite monomer essential for automated oligonucleotide synthesis. This compound is a critical building block for the solid-phase synthesis of DNA and RNA strands, enabling the precise incorporation of modified cytidine bases. It is primarily used by researchers and manufacturers in the fields of therapeutic oligonucleotide development, diagnostic probe synthesis, and academic research to create advanced nucleic acid-based tools and medicines.

Application

• Oligonucleotide Synthesis: A key monomer for the automated solid-phase synthesis of DNA and RNA sequences, particularly those requiring modified cytosine bases.
• Antisense Oligonucleotide (ASO) Manufacturing: Used in the production of therapeutic ASOs for targeting specific mRNA sequences to treat genetic disorders and diseases.
• siRNA and miRNA Synthesis: Essential for constructing short interfering RNA (siRNA) and microRNA (miRNA) strands used in gene silencing and functional genomics research.
• Diagnostic Probe Development: Incorporated into fluorescent probes, molecular beacons, and primers for PCR, sequencing, and in-situ hybridization applications.
• Aptamer and SELEX Library Construction: Used to synthesize randomized or structured nucleic acid libraries for the selection of high-affinity binding molecules (aptamers).
• Research-Grade Oligo Production: Serves as a critical reagent for in-house or contract research organization (CRO) synthesis of custom oligonucleotides for experimental use.

Basic Information

Product Name	5'-Dmt-N4-Ac-5-Me-Dc Phosphoramidite
CAS No.	1873306-74-1
Molecular Formula	C41H51N4O8P
Molecular Weight	758.85 g/mol
Synonyms	5'-O-(4,4'-Dimethoxytrityl)-N4-acetyl-5-methyl-2'-deoxycytidine-3'-[(2-cyanoethyl)-(N,N-diisopropyl)]-phosphoramidite; 5'-DMT-N4-Ac-5-Methyl-dC-CE Phosphoramidite; N4-Acetyl-5-methyl-2'-deoxycytidine-5'-DMT-3'-phosphoramidite; 5-Me-dC(N4-Ac) Phosphoramidite; 5-Methyl-dC Phosphoramidite (N4-Ac, 5'-DMT); 2'-Deoxy-5-methylcytidine, N4-acetyl-5'-O-[bis(4-methoxyphenyl)phenylmethyl]-3'-[(2-cyanoethyl) diisopropylphosphoramidite]; dCMe Phosphoramidite
EINECS	Contact for details

Quality Control

Our 5'-Dmt-N4-Ac-5-Me-Dc Phosphoramidite is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for sensitive oligonucleotide synthesis. Each lot undergoes comprehensive analytical testing, including HPLC, 31P NMR, and 1H NMR, to confirm identity, purity, and suitability for automated synthesis. Certificates of Analysis (COA) with detailed specifications are provided and available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (argon or nitrogen) in a desiccated environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid or foam
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Assay (NMR)	≥ 97.0%
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.