Description

(5-Methyl-2-Oxo-1,3-Dioxol-4-Yl)Methyl 3-((2''-(1H-Diazirin-3-Yl)-[1,1''-Biphenyl]-4-Yl)Methyl)-2-Oxo-2,3-Dihydro-1H-Benzo[D]Imidazole-4-Carboxylate is a sophisticated bifunctional chemical probe designed for advanced photochemical cross-linking and labeling studies. This compound integrates a photoreactive diazirine group with a benzimidazole carboxylate scaffold, enabling precise covalent binding to target biomolecules upon UV light activation. It is primarily utilized by researchers in chemical biology, proteomics, and medicinal chemistry for mapping protein-protein interactions, identifying binding sites, and developing novel activity-based probes.

Application

• Photoaffinity Labeling (PAL): A critical tool for capturing transient or weak biomolecular interactions in live cells or complex mixtures through UV-induced covalent cross-linking.
• Chemical Proteomics: Used to identify and profile protein targets of small molecules, aiding in drug discovery and mechanism-of-action studies.
• Activity-Based Protein Profiling (ABPP): Serves as a probe to monitor enzyme activity and function within a proteome by forming irreversible bonds with active sites.
• Bioconjugation & Linker Chemistry: The reactive handles allow for conjugation to other molecules, surfaces, or reporter tags (e.g., fluorophores, biotin) for detection and purification.
• Structural Biology Studies: Assists in elucidating three-dimensional protein structures and interaction networks by providing covalent anchors for analysis.
• Pharmaceutical R&D: Employed in high-throughput screening and lead optimization to validate drug targets and understand binding kinetics.

Basic Information

Product Name	(5-Methyl-2-Oxo-1,3-Dioxol-4-Yl)Methyl 3-((2''-(1H-Diazirin-3-Yl)-[1,1''-Biphenyl]-4-Yl)Methyl)-2-Oxo-2,3-Dihydro-1H-Benzo[D]Imidazole-4-Carboxylate
CAS No.	1800532-35-7
Molecular Formula	C30H24N4O7
Molecular Weight	552.54 g/mol
Synonyms	Diazirine-Benzimidazole Photoprobe; Bifunctional Diazirine Crosslinker; PAL Probe CAS 1800532-35-7; 4-((5-Methyl-2-oxo-1,3-dioxol-4-yl)methoxycarbonyl)-3-([1,1'-biphenyl]-4-ylmethyl)-1H-benzimidazol-2-one with 3-(2'-diazirinyl) substituent; Photoaffinity Labeling Reagent; Chemical Biology Probe; ABPP Probe; Covalent Inhibitor Scaffold
EINECS	Contact for details

Quality Control

Every batch of this high-value research chemical is synthesized and purified under strict quality-controlled conditions to ensure optimal reactivity and performance in sensitive applications. Standard quality assurance includes verification of photoreactive group integrity and purity analysis. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing key parameters such as purity, identity confirmation, and stability indicators. Our quality system supports research compliance in GLP (Good Laboratory Practice) environments.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a dedicated, moisture-free freezer. Due to its hygroscopic and strictly light-sensitive nature, the product should be handled quickly under subdued light and allowed to equilibrate to room temperature in a desiccator before opening to prevent condensation and degradation. For long-term stability, aliquot into single-use vials.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (IR)	Conforms to structure
Identification (HPLC)	Retention time matches reference
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Solvent Residue (GC)	Meets ICH guidelines
Functional Activity	UV-induced cross-linking efficiency confirmed by control assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.