Description

N1-Methyl-N3-[(2S)-2-(T-Butoxycarbonyl)Amino-3-(T-Butoxycarbonyl)] Propylpseudouridine is a sophisticated, Boc-protected pseudouridine derivative designed for advanced oligonucleotide synthesis. This compound matters because it serves as a critical building block for introducing chemically modified nucleosides into mRNA and other therapeutic nucleic acids, enhancing their stability, reducing immunogenicity, and improving translational efficiency. It is essential for pharmaceutical R&D teams, process development scientists, and CDMOs engaged in the development of next-generation mRNA vaccines, therapeutics, and diagnostic tools.

Application

• mRNA Therapeutic & Vaccine Development: As a key modified nucleoside precursor for codon-optimized mRNA sequences to improve protein expression and pharmacokinetic profiles.
• Antisense Oligonucleotide (ASO) Synthesis: For the solid-phase or solution-phase synthesis of ASOs with enhanced nuclease resistance and target affinity.
• siRNA and miRNA Research: Incorporation into small interfering RNA or microRNA strands to modulate gene silencing activity and specificity.
• Pharmaceutical Intermediate: Serves as a high-purity synthetic intermediate in the multi-step production of active pharmaceutical ingredients (APIs) for nucleic acid-based drugs.
• Process Development & Scale-Up: Used in process chemistry for optimizing synthetic routes and scaling up the production of modified oligonucleotides under GMP-like conditions.
• Academic & Biotech Research: A vital reagent for studying structure-activity relationships (SAR) of nucleic acids and exploring novel mechanisms of action.

Basic Information

Product Name	N1-Methyl-N3-[(2S)-2-(T-Butoxycarbonyl)Amino-3-(T-Butoxycarbonyl)] Propylpseudouridine
CAS No.	1613530-24-7
Molecular Formula	C23H39N3O8
Molecular Weight	485.57 g/mol
Synonyms	1-Methyl-3-[(2S)-2-[(tert-Butoxycarbonyl)amino]-3-[(tert-butoxycarbonyl)oxy]propyl]pseudouridine; Boc-Protected N1-Methylpseudouridine Derivative; (2S)-2-[(tert-Butoxycarbonyl)amino]-3-[(tert-butoxycarbonyl)oxy]propyl 1-Methylpseudouridine; N1-Methyl-N3-[(2S)-2-(Boc-Amino)-3-(Boc-oxy)]propyl-ψ-uridine; Modified Nucleoside Phosphoramidite Precursor; mRNA Modification Building Block; 1613530-24-7
EINECS	Contact for details

Quality Control

Our N1-Methyl-N3-[(2S)-2-(T-Butoxycarbonyl)Amino-3-(T-Butoxycarbonyl)] Propylpseudouridine is manufactured under strict quality systems to ensure batch-to-batch consistency suitable for pharmaceutical research and development. Each lot undergoes comprehensive analytical characterization, including HPLC purity assay, chiral purity verification, and identity confirmation by NMR and mass spectrometry. Certificates of Analysis (COA) detailing all specifications are provided and can be tailored to meet specific project or regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the sealed container to equilibrate to room temperature before opening to minimize moisture absorption. For long-term storage, consider under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 97.0%
Chiral Purity	≥ 99.0% ee
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.