Description

N1-Ethylpseudouridine is a chemically modified nucleoside analog of pseudouridine, a naturally occurring component of RNA. This modification is critical for enhancing the stability and translational efficiency of synthetic mRNA, making it a key building block for advanced therapeutic platforms. It is primarily utilized by biotechnology and pharmaceutical companies engaged in the research, development, and manufacturing of mRNA-based vaccines, therapeutics, and gene editing tools.

Application

• mRNA Vaccine & Therapeutic Manufacturing: A critical raw material for producing modified mRNA with improved stability, reduced immunogenicity, and enhanced protein expression.
• Gene Therapy Research: Used in the synthesis of modified mRNA for in vivo gene editing and protein replacement therapies.
• Cell Reprogramming & Regenerative Medicine: Incorporated into mRNA for the transient expression of reprogramming factors in stem cell research.
• Oncology Research: Employed in the development of mRNA cancer vaccines and immunotherapies.
• Biochemical Research: Serves as a tool compound for studying RNA structure, function, and post-transcriptional modifications.
• Nucleic Acid Chemistry: Used as a monomer in solid-phase oligonucleotide synthesis for research-scale applications.

Basic Information

Product Name	N1-Ethylpseudouridine
CAS No.	1613529-72-8
Molecular Formula	C11H16N2O6
Molecular Weight	272.26 g/mol
Synonyms	1-Ethylpseudouridine; N1-Ethyl-5-ribosyluracil; 5-[(2S,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-1-ethyl-1,2,3,4-tetrahydropyrimidine-2,4-dione; Ethylpseudouridine; N1-Ethyl-ψ; ψ(Et); 1-Ethyl-5-(β-D-ribofuranosyl)uracil; N1-Ethyl-5-ribosyluracil
EINECS	Contact for details

Quality Control

Our N1-Ethylpseudouridine is manufactured under strict quality systems suitable for pharmaceutical and biotechnology applications. Each batch undergoes rigorous analytical testing to ensure identity, purity, and consistency. A comprehensive Certificate of Analysis (COA) detailing specifications such as HPLC purity, water content, residual solvents, and endotoxin levels is provided with every shipment to support your regulatory and quality assurance requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and should be handled in a controlled environment to prevent degradation. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to reference standard
Identification (HPLC)	Conforms to reference standard
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH Q3C guidelines
Endotoxin	< 10 EU/mg
Specific Rotation	Contact for details

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.