Description

N1-Propargyl Pseudouridine is a chemically modified nucleoside analog designed for advanced bioconjugation and labeling applications. This compound is a critical building block in the synthesis of functionalized oligonucleotides, enabling precise chemical tagging and downstream analysis. It is primarily utilized by researchers and manufacturers in the fields of therapeutic oligonucleotide development, diagnostic probe design, and chemical biology.

Application

• Click Chemistry Substrate: The propargyl group serves as a key handle for copper-catalyzed azide-alkyne cycloaddition (CuAAC) reactions, enabling site-specific labeling of RNA.
• Therapeutic Oligonucleotide Synthesis: Used in the production of modified mRNA and other RNA-based therapeutics to incorporate functional groups for tracking or targeting.
• Diagnostic Probe Development: Essential for creating fluorescently labeled or affinity-tagged RNA probes for detection and imaging applications.
• Structural Biology Studies: Incorporation into RNA strands to study RNA-protein interactions, folding, and localization via bioconjugation.
• Chemical Biology Tool: Acts as a versatile reagent for introducing bioorthogonal functionalities into nucleic acids for pull-down assays and cross-linking experiments.
• Precision Medicine Research: Facilitates the development of personalized medicine approaches through tailored oligonucleotide conjugates.

Basic Information

Product Name	N1-Propargyl Pseudouridine
CAS No.	1464021-70-2
Molecular Formula	C12H15N3O6
Molecular Weight	297.26 g/mol
Synonyms	1-Propargylpseudouridine; 5-[(2S,3R,4S,5R)-3,4-Dihydroxy-5-(hydroxymethyl)oxolan-2-yl]-1-(prop-2-yn-1-yl)pyrimidine-2,4-dione; Pseudouridine, 1-propargyl-; N1-Propargyl-5-ribosyluracil; Propargyl Pseudouridine; 1-(2-Propynyl)pseudouridine; N1-Propargyl-ψ; ψ-Propargyl
EINECS	Contact for details

Quality Control

Our N1-Propargyl Pseudouridine is manufactured under strict quality systems to ensure batch-to-batch consistency and high purity suitable for research and pharmaceutical applications. All materials are supported by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to cGMP principles for production and control, ensuring compliance with global regulatory expectations for advanced pharmaceutical intermediates.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and purity. For long-term storage, consider desiccation.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (IR)	Conforms
Assay (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines
Heavy Metals	< 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.