Description

2-(3-But-3-Ynyl-3H-Diazirin-3-Yl)-Ethanol is a specialized diazirine-based chemical building block, a member of the photoaffinity labeling reagents. Its core value lies in its ability to form highly reactive carbene intermediates upon photolysis, enabling the covalent cross-linking and study of biomolecular interactions. This compound is essential for researchers and developers in pharmaceutical discovery, proteomics, and chemical biology who require precise tools for target identification and mapping binding sites.

Application

• Photoaffinity Labeling (PAL): A critical reagent for covalently tagging drug targets, proteins, and receptors upon UV light activation to study molecular interactions.
• Chemical Proteomics: Used in activity-based protein profiling (ABPP) to identify and characterize enzyme active sites and off-target effects of drug candidates.
• Bioconjugation & Probe Synthesis: Serves as a versatile linker for synthesizing bioactive probes, affinity tags, and bifunctional cross-linkers.
• Structural Biology: Aids in the elucidation of protein-ligand complex structures by providing stable cross-links for analysis via mass spectrometry or crystallography.
• Drug Discovery Research: Employed in hit-to-lead optimization to validate binding mechanisms and assess target engagement in complex biological systems.
• Material Science: Potential use in surface functionalization and creating photo-reactive polymeric materials.

Basic Information

Product Name	2-(3-But-3-Ynyl-3H-Diazirin-3-Yl)-Ethanol
CAS No.	1450754-41-2
Molecular Formula	C8H12N2O
Molecular Weight	152.20 g/mol
Synonyms	3-(4-Hydroxybut-1-yn-1-yl)-3H-diazirine; 2-(3-But-3-ynyl-3H-diazirin-3-yl)ethanol; 4-(3H-Diazirin-3-yl)but-3-yn-1-ol; Diazirine alkyne alcohol; Photoaffinity labeling reagent; Carbene precursor; Alkyne-functionalized diazirine
EINECS	Contact for details

Quality Control

Our production of 2-(3-But-3-Ynyl-3H-Diazirin-3-Yl)-Ethanol adheres to stringent quality protocols to ensure batch-to-batch consistency and reliability for sensitive research applications. Each lot is characterized by advanced analytical techniques including NMR, HPLC, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and stability is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a recommended temperature of -20°C or below for long-term stability. The compound is light-sensitive and should be handled under subdued light or in amber glass to prevent premature decomposition. Allow the container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Colorless to pale yellow liquid or low-melting solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95% (Area %)
Water Content (KF)	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.