Description

Cytidine, 4'-C-(Chloromethyl)-2'-Deoxy-3'-o-[(1,1-Dimethylethyl)Dimethylsilyl]-5'-o-[(1,1-Dimethylethyl)Diphenylsilyl]-2'-Fluoro- is a sophisticated, multi-protected nucleoside analog designed for advanced synthetic chemistry. This compound is a critical high-purity building block for the synthesis of modified oligonucleotides, enabling precise structural control in research and development. It is primarily needed by pharmaceutical R&D teams and biotech companies engaged in developing novel therapeutics, including antisense oligonucleotides and siRNA-based drugs.

Application

• Pharmaceutical Intermediate: A key precursor in the solid-phase synthesis of fluorinated and chemically modified oligonucleotides for therapeutic applications.
• Antisense Oligonucleotide (ASO) Development: Used to incorporate specific modifications that enhance nuclease resistance, target affinity, and pharmacokinetic properties of ASO drug candidates.
• siRNA and RNAi Research: Serves as a protected monomer for constructing chemically stabilized siRNA strands to improve delivery and efficacy in gene silencing studies.
• Medicinal Chemistry & Drug Discovery: Enables structure-activity relationship (SAR) studies by allowing precise introduction of a chloromethyl and fluoro group at strategic positions on the nucleoside scaffold.
• Probe and Diagnostic Reagent Synthesis: Utilized in creating labeled nucleotide probes for molecular diagnostics and genomic research.
• Academic Research: Employed in university and institutional labs studying nucleic acid chemistry, enzyme mechanisms, and the development of novel synthetic methodologies.

Basic Information

Product Name	Cytidine, 4'-C-(Chloromethyl)-2'-Deoxy-3'-o-[(1,1-Dimethylethyl)Dimethylsilyl]-5'-o-[(1,1-Dimethylethyl)Diphenylsilyl]-2'-Fluoro-
CAS No.	1445379-90-7
Molecular Formula	C37H52ClFN3O4Si2
Molecular Weight	722.44 g/mol
Synonyms	4'-C-(Chloromethyl)-2'-deoxy-3'-O-[(1,1-dimethylethyl)dimethylsilyl]-5'-O-[(1,1-dimethylethyl)diphenylsilyl]-2'-fluoro-cytidine; 2'-Fluoro-4'-C-chloromethyl-2'-deoxy-3',5'-bis-O-silyl-cytidine; TBDMS/TBDPS Protected 2'-F-4'-C-Chloromethyl Cytidine; 2'-Deoxy-2'-fluoro-4'-C-(chloromethyl)-3'-O-(tert-butyldimethylsilyl)-5'-O-(tert-butyldiphenylsilyl)cytidine; 4'-C-(Chloromethyl)-2'-deoxy-2'-fluoro-3',5'-bis-O-silylcytidine; Advanced Cytidine Monomer for Oligonucleotide Synthesis; Fluorinated & Chloromethylated Protected Nucleoside
EINECS	Contact for details

Quality Control

Our production of this advanced nucleoside intermediate adheres to strict quality protocols to ensure batch-to-batch consistency and high purity essential for sensitive pharmaceutical synthesis. Quality is verified through comprehensive analytical techniques including HPLC, NMR, and mass spectrometry. A detailed Certificate of Analysis (COA) is provided with each shipment, documenting purity, identity, and impurity profiles. We support development under cGMP guidelines where required.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a desiccated environment to prevent degradation due to its hygroscopic (moisture-sensitive) nature. Allow the sealed container to equilibrate to room temperature before opening to minimize moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Meets ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.