Description

N1-Methyl-Pseudouridine-5'-Triphosphate is a chemically modified nucleotide triphosphate analog crucial for advanced mRNA synthesis. This compound is engineered to enhance the stability and translational efficiency of mRNA, addressing key challenges in therapeutic and vaccine development. It is an essential raw material for biotechnology and pharmaceutical companies engaged in cutting-edge mRNA technology, including the production of next-generation vaccines, protein replacement therapies, and immuno-oncology treatments.

Application

• mRNA Vaccine Production: A key component for synthesizing modified mRNA with improved stability and reduced immunogenicity for prophylactic and therapeutic vaccines.
• Therapeutic mRNA Development: Used in the research and manufacturing of mRNA-based drugs for protein replacement, gene editing, and cancer immunotherapy.
• In Vitro Transcription (IVT): Serves as a modified UTP substitute in enzymatic RNA synthesis to produce mRNA with enhanced properties for research and clinical applications.
• Biochemical Research: A critical reagent for studying RNA structure, function, and metabolism, as well as for developing novel RNA-based diagnostic tools.
• Cell-Free Protein Synthesis Systems: Incorporated into platforms for high-yield, cell-free production of difficult-to-express proteins.

Basic Information

Product Name	N1-Methyl-Pseudouridine-5'-Triphosphate
CAS No.	1428903-59-6
Molecular Formula	C11H18N3O14P3
Molecular Weight	521.19 g/mol
Synonyms	1-Methylpseudouridine-5'-triphosphate; m1ψTP; N1-Methyl-ψ-5'-TP; 5'-Triphosphoric acid 1-methylpseudouridine; 1-Methyl-5-(triphospho-β-D-ribofuranosyl)pseudouracil; N1-Methyl-5-(trihydroxyphosphoryloxyphosphorylphosphonooxymethyl)-2,4(1H,3H)-pyrimidinedione; Modified Nucleotide Triphosphate; m1ψ-5'-Triphosphate
EINECS	Contact for details

Quality Control

Our N1-Methyl-Pseudouridine-5'-Triphosphate is manufactured under strict quality systems suitable for pharmaceutical and research applications. Each batch is subjected to rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets stringent specifications for use in sensitive mRNA applications. Certificates of Analysis (COA) with detailed chromatographic data are provided to guarantee traceability, consistency, and compliance with your project requirements.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0%
Triphosphate Content	≥ 85%
Endotoxin	< 10 EU/mg
Biological Activity (IVT)	Functional

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.