Description

2'-o-tert-Butyldimethylsilyl-5-Ethinyl Uridine is a key protected nucleoside derivative, specifically designed for advanced oligonucleotide synthesis and medicinal chemistry research. This compound is critical for introducing modified bases into nucleic acid strands, enabling the development of stable and targeted therapeutic agents. It is primarily utilized by researchers and manufacturers in the pharmaceutical and biotechnology sectors for the development of antisense oligonucleotides, siRNA, and other novel nucleic acid-based therapeutics.

Application

• Oligonucleotide Synthesis: Serves as a crucial building block for the solid-phase synthesis of modified RNA and DNA strands, particularly where ethynyl modifications are required.
• Antisense Drug Development: Used in the research and production of antisense oligonucleotides (ASOs) designed to target specific mRNA sequences.
• siRNA and miRNA Therapeutics: A key intermediate for creating stabilized small interfering RNA (siRNA) and microRNA (miRNA) constructs with enhanced nuclease resistance.
• Medicinal Chemistry & Prodrug Design: Employed in the synthesis of nucleoside prodrugs and analogs for antiviral and anticancer research.
• Bioconjugation and Labeling: The ethynyl group allows for efficient click chemistry (e.g., CuAAC) conjugation with azide-containing probes, dyes, or delivery vehicles.
• Academic and Industrial R&D: A vital reagent for universities and contract research organizations (CROs) exploring novel nucleic acid chemistries and functions.

Basic Information

Item	Details
Product Name	2'-o-tert-Butyldimethylsilyl-5-Ethinyl Uridine
CAS No.	1428477-12-6
Molecular Formula	C17H28N2O6Si
Molecular Weight	384.51 g/mol
Synonyms	5-Ethynyl-2'-O-(tert-butyldimethylsilyl)uridine; 2'-O-TBDMS-5-ethynyluridine; 5-Ethynyl-2'-O-[(1,1-dimethylethyl)dimethylsilyl]uridine; 5-Ethynyl-2'-O-(TBDMS)uridine; TBDMS-5-Ethynyluridine; 2'-O-tert-Butyldimethylsilyl-5-ethynyluridine; 5-Ethynyl-2'-O-silyluridine (TBDMS protected)
EINECS	Contact for details

Quality Control

Every batch of 2'-o-tert-Butyldimethylsilyl-5-Ethinyl Uridine is manufactured under controlled conditions and undergoes rigorous analytical testing to ensure identity, purity, and consistency. Our quality system is designed to meet the stringent requirements of research and pre-clinical development. A comprehensive Certificate of Analysis (COA) detailing purity (by HPLC), identity (by NMR and MS), and other critical parameters is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability. For long-term storage, consider storing under an inert atmosphere such as argon or nitrogen.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 1.0%
Residual Solvents (GC)	Complies with ICH guidelines

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.